Oligonucleotide Probes

Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.

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Networked: 387 relevant articles (2 outcomes, 34 trials/studies)

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Bio-Agent Context: Research Results

Experts

1.	Laassri, Majid: 2 articles (05/2011 - 09/2003)
2.	Chumakov, Konstantin: 2 articles (05/2011 - 09/2003)
3.	Yokoi, Kiyoshi: 2 articles (06/2010 - 05/2010)
4.	Yamada, Yoshiji: 2 articles (06/2010 - 05/2010)
5.	Fujimaki, Tetsuo: 2 articles (06/2010 - 05/2010)
6.	Satoh, Kei: 2 articles (06/2010 - 05/2010)
7.	Yoshida, Hidemi: 2 articles (06/2010 - 05/2010)
8.	Watanabe, Sachiro: 2 articles (06/2010 - 05/2010)
9.	Yoshida, Tetsuro: 2 articles (06/2010 - 05/2010)
10.	Oguri, Mitsutoshi: 2 articles (06/2010 - 05/2010)

Related Diseases

1.	Neoplasms (Cancer) 10/01/2003 - "By arraying oligonucleotide probes designed from the human genome sequence, and hybridizing with "representations" from cancer and normal cells, we detect regions of the genome with altered "copy number." We achieve an average resolution of 30 kb throughout the genome, and resolutions as high as a probe every 15 kb are practical. " 07/01/1996 - "This method required neither determination of nucleotide sequences nor construction of tumor-specific oligonucleotide probes or primers. " 11/11/1993 - "Ambiguous signals recorded with oligonucleotide probes were further analyzed using SSCP analysis revealing the presence of H-ras mutations in restricted regions of some tumors. " 06/01/1993 - "Nonspecific in situ hybridization reaction in neuroendocrine cells and tumors of the gastrointestinal tract using oligonucleotide probes." 03/01/1993 - "In the analysis of tumor cell lines and tumor samples, the results were identical with those of conventional dot blot hybridization using 32P-labeled oligonucleotide probes. "
2.	Infection 01/01/2011 - "In this study, genes that are differentially expressed in the caudal medulla tissues of animals infected with different doses of PrP(BSE) at 12 and 45 mo post infection were compared using array containing 24,000 oligonucleotide probes. " 10/01/1999 - "Due to the potential ability of short, labeled, oligonucleotide probes to specifically distinguish between different phytoplasmas present in multiple infections, this technique should provide a powerful new tool for epidemiological and vector ecology studies." 03/01/1995 - "This study confirms the potential of oligonucleotide probes for detecting and typing HPV infections." 09/01/1992 - "An in situ hybridization method using radiolabeled oligonucleotide probes was developed to study primary sites of hepatitis A virus replication in an experimental animal model of infection. " 10/01/2013 - "Two newly designed oligonucleotide probes specific for previously identified Theileria spp. of giraffes found single infections in eight of the specimens and mixed infections in the remaining five samples. "
3.	beta-Thalassemia (Cooley's Anemia) 04/01/1991 - "During the molecular study of the Chinese beta thalassemia, we encountered a mutation that could not be detected by any of the oligonucleotide probes specific for the known Chinese beta thalassemia mutations. " 10/01/2002 - "Reverse dot-blot hybridization using oligonucleotide probes specific for Southeast Asian mutations can detect beta-thalassemia in 60 chromosomes in addition to 31 chromosomes with beta(E) mutation. " 01/01/1995 - "Prenatal diagnosis for beta-thalassemia syndromes using HRP-labeled oligonucleotide probes at Siriraj Hospital." 02/01/1992 - "Three thousand beta-thalassemia chromosomes from prospective parents presenting at the genetic service were initially analyzed by dot blot analysis with oligonucleotide probes complementary to the most common beta-thalassemia mutations in the Mediterranean at-risk populations. " 04/01/1990 - "We studied 150 chromosomes of 95 beta-thalassemia patients from Guangxi, Guangdong and Sichuan Provinces using the polymerase chain reaction followed by dot hybridization with specific oligonucleotide probes. "
4.	Carcinoma (Carcinomatosis) 06/01/1997 - "This study aims to establish the prevalence of HHV-6A and HHV-6B in carcinoma tissues using variant-specific oligonucleotide probes. " 06/01/1993 - "Oligonucleotide probes based on the human kidney TPMT amino acid sequence were then used to screen a T84 human colon carcinoma cell cDNA library. " 12/15/2004 - "Nine sets of oligonucleotide probes were designed to fabricate a DNA microarray to detect the methylation changes of p16 gene CpG islands in gastric carcinomas. " 09/01/1996 - "In situ hybridization using EBERs and BHLF oligonucleotide probes and immunohistochemistry using monoclonal antibodies against LMP1, EBNA2, BZLF1 protein, p53 protein and bcl-2 protein were performed on 56 primary nasopharyngeal carcinomas. " 03/01/1994 - "We have developed oligonucleotide probes for each of the six non-neuronal human MT (hMT) isoforms and used them to assay hMT isoform expression in three pairs of CDDP-resistant and -sensitive human carcinoma cell lines, i.e., SCC25/CP versus SCC25 cells, H69/CP versus H69 cells, and SW2/CP versus SW2 cells. "
5.	Adenoma (Adenomas) 10/01/1997 - "We studied the expression of CaR mRNA in pairs of adenoma and adenoma-associated normal gland from the same patients (n = 17) and in biopsies of normal parathyroid glands of normocalcemic subjects (n = 4) using in situ hybridization with oligonucleotide probes on frozen sections. " 08/01/1990 - "Northern hybridization analysis with the oligonucleotide probes in 2 normal pituitaries and an adenoma causing Cushing's disease detected a 1.2-Kb mRNA in all three tissues, indicating that the oligonucleotide probes were very specific. " 03/01/1989 - "To examine this question, in situ hybridization with oligonucleotide probes was done on routinely processed tissues received in the pathology laboratory to analyze for the presence of GH and PRL messenger RNA (mRNA) in 4 normal pituitaries, 10 prolactinomas, and 16 GH-secreting adenomas. " 01/01/2001 - "This study aimed at demonstrating mRNAs for all main pituitary hormones in 32 null cell adenomas and 31 oncocytomas by non-isotopic in situ hybridization using digoxigenin-labeled oligonucleotide probes. " 01/01/2010 - "DNA extracted from paraffin blocks of six metanephric adenomas was hybridized onto Agilent oligoarrays with approximately 43,000 in situ synthesized 60-mer oligonucleotide probes that span coding and non-coding sequences with an average spatial resolution of approximately 35 kb. None of the metanephric adenomas showed DNA copy number changes. "

Related Drugs and Biologics

1.	Biotin (Vitamin H)
2.	RNA (Ribonucleic Acid)
3.	Messenger RNA (mRNA)
4.	DNA (Deoxyribonucleic Acid)
5.	Digoxigenin
6.	Cytokines
7.	Codon (Codons)
8.	Paraffin
9.	Interleukin-6 (Interleukin 6)
10.	Tumor Necrosis Factor-alpha (Tumor Necrosis Factor)

Related Therapies and Procedures

1.	Transplants (Transplant)
2.	Bone Marrow Transplantation (Transplantation, Bone Marrow)
3.	Electrodes (Electrode)
4.	Stents
5.	Heterologous Transplantation (Xenotransplantation)