Oligonucleotide Probes

Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.

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Networked: 641 relevant articles (3 outcomes, 48 trials/studies)

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Bio-Agent Context: Research Results

Experts

1.	Sumana, Gajjala: 4 articles (09/2016 - 04/2012)
2.	Sapra, Sameer: 3 articles (07/2013 - 04/2012)
3.	Sharma, Aditya: 3 articles (07/2013 - 04/2012)
4.	Ducho, Christian: 2 articles (01/2020 - 07/2019)
5.	Fischer, Bilha: 2 articles (01/2020 - 07/2019)
6.	Saady, Abed: 2 articles (01/2020 - 07/2019)
7.	Astakhova, Kira: 2 articles (12/2018 - 01/2015)
8.	Imagawa, Tomoyuki: 2 articles (01/2017 - 03/2011)
9.	Kikuchi, Masako: 2 articles (01/2017 - 03/2011)
10.	Miyamae, Takako: 2 articles (01/2017 - 03/2011)

Related Diseases

1.	Neoplasms (Cancer) 01/01/2016 - "For the DHP-based colorimetric sensing system, as a model analyte, cancer-related DNA sequence can trigger a cascade polymerization/nicking cycle on only one oligonucleotide probe. " 01/01/2011 - "Evaluation of an 18F-labeled oligonucleotide probe targeting p21(WAF1) transcriptional changes in human tumor cells." 10/01/2003 - "By arraying oligonucleotide probes designed from the human genome sequence, and hybridizing with "representations" from cancer and normal cells, we detect regions of the genome with altered "copy number." We achieve an average resolution of 30 kb throughout the genome, and resolutions as high as a probe every 15 kb are practical. " 08/01/1997 - "The EBV genome was detected by PCR using primers targeting the IR region in three (6.8%) of the CIN I/II, 15 (21.4%) of the CIN III and 13 (21.7%) of the ISCC lesions, while using an EBER oligonucleotide probe RISH revealed positive signals in infiltrating lymphocytes located in the cervical stroma, but not in dysplastic or tumor cells. " 07/01/1996 - "This method required neither determination of nucleotide sequences nor construction of tumor-specific oligonucleotide probes or primers. "
2.	Inborn Genetic Diseases (Disease, Hereditary) 05/01/1991 - "The improved sensitivity obtained by chemiluminescent detection should increase the usefulness of oligonucleotide probes in the direct Southern analysis of human genetic disorders." 03/15/2013 - "The proof-of-concept scheme is expected to provide new insight into the potential of G-quadruplex structure and promote the application of fluorescent oligonucleotide probes in fundamental research, diagnosis, and treatment of genetic diseases." 01/01/2011 - "This method was tested by capturing sequences from a DNA library through hybridization to oligonucleotide probes specific for genetic disorder-related genes and was found to show high selectivity, improve the detection of mutations, enabling the discovery of novel variants, and provide additional indel data. "
3.	Infections 01/01/2011 - "In this study, genes that are differentially expressed in the caudal medulla tissues of animals infected with different doses of PrP(BSE) at 12 and 45 mo post infection were compared using array containing 24,000 oligonucleotide probes. " 10/01/1999 - "Due to the potential ability of short, labeled, oligonucleotide probes to specifically distinguish between different phytoplasmas present in multiple infections, this technique should provide a powerful new tool for epidemiological and vector ecology studies." 09/01/1992 - "An in situ hybridization method using radiolabeled oligonucleotide probes was developed to study primary sites of hepatitis A virus replication in an experimental animal model of infection. " 07/01/2008 - "To investigate endothelial cells and other potentially cryptic sites of Anaplasma sp. infection in mammalian tissues, a sensitive and specific isothermal in situ technique to detect localized Anaplasma gene sequences by using rolling-circle amplification of circularizable, linear, oligonucleotide probes (padlock probes) was developed. " 01/01/2005 - "Using genospecies-specific oligonucleotide probes, single Borrelia genospecies infections (91.4%) could be assigned to B. "
4.	Rheumatoid Arthritis 01/01/1995 - "We have analyzed HLA class II alleles in a group of 153 Czech children with rheumatoid arthritis by PCR and hybridization with oligonucleotide probes. " 04/01/1993 - "Seventy-two patients with rheumatoid arthritis (RA) and 82 controls have been typed with the XI Histocompatibility Workshop DRB1 and DQB1 sequence-specific oligonucleotide probes. " 02/01/1993 - "To reveal immunogenetic factors involved in the pathogenesis of rheumatoid arthritis(RA), two hundreds and four unrelated Japanese patients with RA were typed for HLA by both serologic typing and DNA typing using polymerase chain reaction-sequence specific oligonucleotide probe (PCR-SSOP) method. " 01/01/1989 - "We examined the association of individual HLA genes with rheumatoid arthritis (RA), using oligonucleotide probes that identified both DR4-associated and non-DR4-associated genes. " 01/01/2010 - "In this paper an assay for the detection of genes associated with rheumatoid arthritis (RA) and multiple sclerosis, using polymerase chain reaction (PCR) and sequence specific oligonucleotide probes (SSOP) is presented, in order to be further applied in a portable Lab-On-Chip (LOC) device. "
5.	Adenoma (Adenomas) 10/01/1997 - "We studied the expression of CaR mRNA in pairs of adenoma and adenoma-associated normal gland from the same patients (n = 17) and in biopsies of normal parathyroid glands of normocalcemic subjects (n = 4) using in situ hybridization with oligonucleotide probes on frozen sections. " 01/01/1999 - "In situ hybridization studies with oligonucleotide probes showed positive staining in all groups with stronger staining in GH, ACTH, TSH, and gonadotroph adenomas and in pituitary carcinomas. " 08/01/1990 - "Northern hybridization analysis with the oligonucleotide probes in 2 normal pituitaries and an adenoma causing Cushing's disease detected a 1.2-Kb mRNA in all three tissues, indicating that the oligonucleotide probes were very specific. " 03/01/1989 - "To examine this question, in situ hybridization with oligonucleotide probes was done on routinely processed tissues received in the pathology laboratory to analyze for the presence of GH and PRL messenger RNA (mRNA) in 4 normal pituitaries, 10 prolactinomas, and 16 GH-secreting adenomas. " 01/01/2001 - "This study aimed at demonstrating mRNAs for all main pituitary hormones in 32 null cell adenomas and 31 oncocytomas by non-isotopic in situ hybridization using digoxigenin-labeled oligonucleotide probes. "

Related Drugs and Biologics

1.	RNA (Ribonucleic Acid)
2.	Biotin (Vitamin H)
3.	Coloring Agents (Dyes)
4.	DNA (Deoxyribonucleic Acid)
5.	Messenger RNA (mRNA)
6.	Proteins (Proteins, Gene)
7.	Digoxigenin
8.	Cytokines
9.	Epstein-Barr virus encoded RNA 1
10.	Suspensions

Related Therapies and Procedures

1.	Bone Marrow Transplantation (Transplantation, Bone Marrow)
2.	Electrodes (Electrode)
3.	Ligation
4.	Transplantation
5.	Stents