DNA Probes

Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.

Also Known As:

Networked: 1031 relevant articles (6 outcomes, 102 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1.	Liu, S: 4 articles (12/2000 - 01/2000)
2.	Attia, Sabry M: 3 articles (06/2013 - 10/2011)
3.	Diebold, J: 3 articles (09/2010 - 04/2000)
4.	Vorsanova, Svetlana G: 3 articles (05/2009 - 03/2005)
5.	Iourov, Ivan Y: 3 articles (05/2009 - 03/2005)
6.	Yurov, Yuri B: 3 articles (05/2009 - 03/2005)
7.	Vorsanova, S G: 3 articles (09/2006 - 01/2001)
8.	Schmid, T E: 3 articles (06/2004 - 01/2001)
9.	Jia, D: 3 articles (12/2000 - 08/2000)
10.	Zhang, Z: 3 articles (12/2000 - 08/2000)

Related Diseases

1.	Infection 01/01/2010 - "DNA probes were used as the gold standard definition for N gonorrhoeae and C trachomatis infection. " 12/01/2001 - "In the current study we present measures of maternal periodontal infection using whole chromosomal DNA probes to identify 15 periodontal organisms within maternal periodontal plaque sampled at delivery. " 05/01/1995 - "Studies using other HHV-6 DNA probes suggested infection with HHV-6 variant B in both cases. " 10/01/2003 - "To evaluate the transplacental transfer of Theileria sergenti infection in cattle, we used DNA probes to detect T. " 06/01/2002 - "DNA probes were used in in situ hybridisation on histological sections of oysters exposed for defined intervals to Marteilia sydneyi infection to reveal the early development of the parasite in the oyster host, Saccostrea glomerata. "
2.	Neoplasms (Cancer) 01/15/1990 - "Using in situ hybridization with 3H-labeled DNA probes nine of the 16 cancers gave positive results with HPV 16. " 01/01/2009 - "We undertook a molecular cytogenetic study of tumor specimens obtained from seven patients using fluorescence in situ hybridization (FISH) with DNA probes mapping to chromosome 1p36, 1q25, 19p13 and 19q13. " 01/01/1994 - "These data indicate that fluorescence in situ hybridization with chromosome-specific repetitive DNA probes can serve as a cytogenetic tool for the analysis of interphase nuclei of solid human tumors and may be useful for the study of tumor cell heterogeneity." 01/01/2013 - "In recent years, FISH has also become automated to accommodate the increased volume of slide preparations necessary for the number of DNA probes needed to detect characteristic molecular anomalies in cancer tissues and bone marrow samples. " 09/01/2011 - "More cases with long-term follow up and greater numbers of DNA probes are necessary to extend this conclusion to other ambiguous melanocytic tumors."
3.	Down Syndrome (Down's Syndrome) 01/01/1990 - "Methods based on using region-specific chromosome 21 DNA probes and fluorescence in situ hybridization show promise for the rapid diagnosis of trisomy 21 (Lichter et al., 1988). " 01/01/1990 - "The continued development of chromosome 21 DNA probes and advances in the technology of molecular cytogenetics will facilitate the study of the genetic organization of chromosome 21 and its role in the pathogenesis of Down syndrome." 01/01/1990 - "Detection of Down syndrome by in situ hybridization with chromosome 21 specific DNA probes." 08/01/1989 - "We could not analyze the region between loci D21S58 and D21S55 and between COL6A1 and 21qter at the molecular level due to a lack of DNA probes and, consequently, the contribution of these areas to a Down syndrome phenotype when present in three copies is unknown. " 02/01/1988 - "The use of DNA probes to establish parental origin in Down syndrome."
4.	Communicable Diseases (Infectious Diseases) 08/01/1994 - "Use of DNA probes in infectious diseases." 11/01/1992 - "Selected current applications of DNA probes in the area of infectious diseases, genetic diseases, HLA typing, paternity and forensic testing are briefly discussed. " 05/01/1992 - "The fundamental aspects of DNA probes and their clinical applications are reviewed to provide the clinician new information on the recent progress in infectious diseases." 01/01/1991 - "[DNA probes instead of culture methods in diagnosis of infectious diseases?]." 11/01/1990 - "Also he reviews gene's function and constitution, DNA probes genoma, genetic code, gene mapping and others current problems in genetics: diagnostic applications, infectious diseases, prevention of inherited diseases, genetic counseling, prenatal sex determination by DNA probes, cell gene therapy and finally the ethical issues."
5.	Acute Myeloid Leukemia (Acute Myelogenous Leukemia) 12/15/1991 - "We have characterized this region in a 5q- patient with therapy-related ANLL (t-ANLL) by pulsed-field gel electrophoresis and Southern blotting analysis utilizing DNA probes for PDGFR, c-fms, and FGFA. " 06/01/2013 - "Combined immunophenotyping and fluorescence in situ hybridization with chromosome-specific DNA probes allows quantification and differentiation of ex vivo generated dendritic cells, leukemia-derived dendritic cells and clonal leukemic cells in patients with acute myeloid leukemia." 06/15/1992 - "We studied the efficacy of fluorescence in situ hybridization (FISH) detection of trisomy 8 in patients with MPD, MDS, or ANLL using directly labeled fluorescent alpha-satellite and whole chromosome paint (WCP) DNA probes specific for chromosome 8. Using FISH, we analyzed interphase nuclei and metaphase spreads from randomized series of BM specimens from normal individuals and patients with varying proportions of trisomy 8 as determined by conventional cytogenetic analysis. " 12/01/1995 - "We have developed sets of genomic DNA probes for the identification of chromosome aberrations associated with chronic lymphocytic leukemia (CLL), chronic myeloid leukemias (CML), and acute myeloid leukemia (AML). " 05/01/1993 - "Using appropriate DNA probes, the configurations of the T-cell receptor beta-chain genes and immunoglobulin heavy-chain genes were studied in patients diagnosed as having the following malignancies: 7 chronic myeloid leukemia, 13 acute myeloblastic leukemia, 9 acute lymphocytic leukemia and 20 chronic lymphocytic leukemia. "

Related Drugs and Biologics

1.	Monoclonal Antibodies
2.	Radioisotopes (Radionuclides)
3.	trisomy Chromosome 8
4.	DNA (Deoxyribonucleic Acid)
5.	Paraffin
6.	Genetic Markers (Genetic Marker)
7.	Factor VIII (Coagulation Factor VIII)
8.	Fluorescent Dyes (Fluorescent Probes)
9.	Formaldehyde (Formol)
10.	Biotin (Vitamin H)

Related Therapies and Procedures

1.	Transplants (Transplant)
2.	Drug Therapy (Chemotherapy)
3.	Electrodes (Electrode)
4.	Examination Tables
5.	Laser Therapy (Surgery, Laser)