DNA Probes

Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Also Known As:
Conserved Gene Probes; DNA Hybridization Probes; Whole Chromosomal Probes; Whole Genomic DNA Probes; Chromosomal Probes, Whole; DNA Gene Probes; Gene Probes, Conserved; Hybridization Probes, DNA; Probes, Chromosomal; Probes, Conserved Gene; Probes, DNA; Probes, DNA Gene; Probes, DNA Hybridization; Probes, Whole Chromosomal; Chromosomal Probes; Gene Probes, DNA
Networked: 1031 relevant articles (6 outcomes, 102 trials/studies)

Relationship Network

Bio-Agent Context: Research Results


1. Liu, S: 4 articles (12/2000 - 01/2000)
2. Attia, Sabry M: 3 articles (06/2013 - 10/2011)
3. Diebold, J: 3 articles (09/2010 - 04/2000)
4. Vorsanova, Svetlana G: 3 articles (05/2009 - 03/2005)
5. Iourov, Ivan Y: 3 articles (05/2009 - 03/2005)
6. Yurov, Yuri B: 3 articles (05/2009 - 03/2005)
7. Vorsanova, S G: 3 articles (09/2006 - 01/2001)
8. Schmid, T E: 3 articles (06/2004 - 01/2001)
9. Jia, D: 3 articles (12/2000 - 08/2000)
10. Zhang, Z: 3 articles (12/2000 - 08/2000)

Related Diseases

1. Infection
2. Neoplasms (Cancer)
3. Down Syndrome (Down's Syndrome)
4. Communicable Diseases (Infectious Diseases)
5. Acute Myeloid Leukemia (Acute Myelogenous Leukemia)
12/15/1991 - "We have characterized this region in a 5q- patient with therapy-related ANLL (t-ANLL) by pulsed-field gel electrophoresis and Southern blotting analysis utilizing DNA probes for PDGFR, c-fms, and FGFA. "
06/01/2013 - "Combined immunophenotyping and fluorescence in situ hybridization with chromosome-specific DNA probes allows quantification and differentiation of ex vivo generated dendritic cells, leukemia-derived dendritic cells and clonal leukemic cells in patients with acute myeloid leukemia."
06/15/1992 - "We studied the efficacy of fluorescence in situ hybridization (FISH) detection of trisomy 8 in patients with MPD, MDS, or ANLL using directly labeled fluorescent alpha-satellite and whole chromosome paint (WCP) DNA probes specific for chromosome 8. Using FISH, we analyzed interphase nuclei and metaphase spreads from randomized series of BM specimens from normal individuals and patients with varying proportions of trisomy 8 as determined by conventional cytogenetic analysis. "
12/01/1995 - "We have developed sets of genomic DNA probes for the identification of chromosome aberrations associated with chronic lymphocytic leukemia (CLL), chronic myeloid leukemias (CML), and acute myeloid leukemia (AML). "
05/01/1993 - "Using appropriate DNA probes, the configurations of the T-cell receptor beta-chain genes and immunoglobulin heavy-chain genes were studied in patients diagnosed as having the following malignancies: 7 chronic myeloid leukemia, 13 acute myeloblastic leukemia, 9 acute lymphocytic leukemia and 20 chronic lymphocytic leukemia. "

Related Drugs and Biologics

1. Monoclonal Antibodies
2. Radioisotopes (Radionuclides)
3. trisomy Chromosome 8
4. DNA (Deoxyribonucleic Acid)
5. Paraffin
6. Genetic Markers (Genetic Marker)
7. Factor VIII (Coagulation Factor VIII)
8. Fluorescent Dyes (Fluorescent Probes)
9. Formaldehyde (Formol)
10. Biotin (Vitamin H)

Related Therapies and Procedures

1. Transplants (Transplant)
2. Drug Therapy (Chemotherapy)
3. Electrodes (Electrode)
4. Examination Tables
5. Laser Therapy (Surgery, Laser)