RNA-Directed DNA Polymerase (Reverse Transcriptase)

An enzyme that synthesizes DNA on an RNA template. It is encoded by the pol gene of retroviruses and by certain retrovirus-like elements. EC 2.7.7.49.

Also Known As:

Reverse Transcriptase; RNA Transcriptase; Revertase; DNA Polymerase, RNA Directed; DNA Polymerase, RNA-Dependent; RNA Dependent DNA Polymerase; RNA Directed DNA Polymerase; DNA Polymerase, RNA-Directed; RNA-Dependent DNA Polymerase; Deoxynucleotide-triphosphate:DNA deoxynucleotidyltransferase (RNA-directed)

Networked: 8825 relevant articles (182 outcomes, 868 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1.	Hoon, Dave S B: 14 articles (02/2022 - 12/2002)
2.	Nuovo, Gerard J: 12 articles (06/2014 - 09/2002)
3.	Mori, Masaki: 11 articles (05/2014 - 03/2007)
4.	Spadafora, Corrado: 10 articles (01/2020 - 06/2005)
5.	Wang, Ying: 10 articles (01/2016 - 04/2004)
6.	Wang, Y: 9 articles (11/2021 - 06/2000)
7.	Li, Li: 9 articles (11/2021 - 01/2002)
8.	Wang, Jian: 9 articles (02/2014 - 01/2004)
9.	Oshima, Takashi: 9 articles (10/2012 - 05/2008)
10.	Brabender, Jan: 9 articles (09/2010 - 06/2004)

Related Diseases

1.	COVID-19 05/06/2021 - "Patients with the highly suggestive symptoms who had computed tomography (CT) imaging results with typical findings of COVID-19 or positive results of reverse transcriptase-polymerase chain reaction (RT-PCR) were enrolled in the study. " 01/01/2023 - "The gold standard test for diagnosing COVID-19 is real-time reverse transcriptase-polymerase chain reaction (RT-PCR), which necessitates highly skilled workers and has a lengthy turnaround time. " 10/01/2022 - "The performance of a new point-of-care CE-IVD-marked isothermal lab-on-phone COVID-19 assay was assessed in comparison to a gold standard real-time reverse transcriptase-PCR method. " 03/07/2023 - "The high sensitivity of reverse transcriptase-polymerase chain reaction (RT-PCR) assay is the gold standard for the diagnosis of COVID-19, but there are still some false-negative results. " 01/01/2020 - "Currently, the gold standard for test is Reverse Transcriptase Polymerase Chain Reaction (RT-PCR); however, patients with a high clinical suspicion for COVID-19 can sometimes have multiple negative tests. "
2.	Infections 03/01/2022 - "The gold standard for diagnosing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is microbiological confirmation by reverse transcriptase-polymerase chain reaction (RT-PCR)1 most commonly done using oropharyngeal (OP) and nasopharyngeal swabs (NP). " 01/01/2001 - "This agent was highly effective against HIV replication regardless of the addition period during early stages of infection (adsorption to integration) but did not inhibit reverse transcriptase activity directly. " 01/01/2023 - "While Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) represents the diagnostic gold standard of infection, computed tomography (CT) has been shown to have an important role in supporting the diagnosis, quantifying the severity, and assessing the efficacy of treatment and its response. " 02/01/2023 - "Although real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR) remains as a golden standard for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, it can not be easily expanded to large-scaled screening during outbreaks, and the positive results do not necessarily correlate with infectious status of the identified subjects. " 01/01/2023 - "Reverse-transcriptase (RT) quantitative polymerase chain reaction (qPCR) assays are the current gold-standard in diagnosis and serotyping of infections, but their use in low-middle income countries (LMICs) has been limited by laboratory infrastructure requirements. "
3.	Neoplasms (Cancer) 05/01/2000 - "Molecular techniques, such as reverse-transcriptase polymerase chain reaction, may be useful tools to reach this target, but, today, immunocytochemistry is still considered the gold standard to assess new techniques to detect isolated tumor cells in hematopoietic tissue. " 05/01/2012 - "Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was conducted to analyze the expressions of miR-155 and its potential target genes in paired tumor tissues and adjacent tumor-free tissues and in disease-free liver tissue samples. " 01/01/1999 - "Several reports suggest that reverse transcriptase polymerase chain reaction (RT-PCR) holds great promise in the early staging of cancer patients in general. " 01/01/2022 - "After IC50 treatments, the anti-cancer effects were evaluated via cell migration assay, cell cycle analysis and apoptotic studies which included histochemical staining and reverse-transcriptase polymerase chain reaction (RT-PCR) of the apoptotic genes. " 03/01/2021 - "High clinical suspicion ought to supersede false-negative NP reverse transcriptase-PCR as early bronchoscopic evaluation in cancer patients, who are either receiving active treatment or are immunosuppressed, can allow timely institution of efficacious treatment, enrollment into clinical trials, as well as effective infection control. "
4.	Human Influenza (Influenza) 06/02/2010 - "A duplex real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay was improved for simultaneous detection of highly pathogenic H5N1 avian influenza virus and pandemic H1N1 (2009) influenza virus, which is suitable for early diagnosis of influenza-like patients and for epidemiological surveillance. " 04/01/2011 - "We assessed the test characteristics of Influenzatop®, a rapid immunochromatographic influenza A and B test, in detecting pandemic 2009 influenza A (H1N1) in children up to 18 years of age, using reverse transcriptase polymerase chain reaction (RT-PCR) as the gold standard. " 12/01/2012 - "Using the reverse transcriptase polymerase chain reaction as a gold standard, the overall sensitivity (0.57) was higher than that of the QuickVue Influenza A+B Test (0.43). " 09/01/2012 - "Patients were defined as being influenza-positive if the real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) test had positive results. " 05/01/2012 - "Although real-time reverse transcriptase-polymerase chain reactions (rRT-PCR) are gold standard for the diagnosis of influenza A (H1N1), the results can be delayed up to several days, which is problematic during a pandemic. "
5.	Melanoma (Melanoma, Malignant) 01/01/2021 - "Positive results of reverse transcriptase polymerase chain reaction (RT-PCR) test have a strong relationship with melanoma recurrence and disease-specific survival time in stage III melanoma." 02/01/2012 - "The objective is to evaluate the efficacy of SLNB in Japanese patients with primary cutaneous melanoma and to elucidate whether reverse transcriptase (RT)-PCR analysis of sentinel lymph nodes (SLNs) is valuable for predicting patient outcome. " 07/01/1999 - "The purpose of this study was to assess the prognostic significance of the detection of circulating melanoma cells by reverse transcriptase-PCR in long-term clinically disease-free melanoma patients. " 04/15/2000 - "We assessed the prognostic clinical utility of a multimarker melanoma reverse transcriptase-PCR (RT-PCR) assay using blood of 46 patients who were clinically disease-free. " 07/01/1999 - "Prognostic significance of the detection of circulating malignant cells by reverse transcriptase-polymerase chain reaction in long-term clinically disease-free melanoma patients."

Related Drugs and Biologics

1.	Messenger RNA (mRNA)
2.	RNA (Ribonucleic Acid)
3.	Vaccines
4.	Antigens
5.	DNA (Deoxyribonucleic Acid)
6.	Nucleic Acids
7.	Immunoglobulin M (IgM)
8.	Protease Inhibitors (Protease Inhibitor)
9.	Proteins (Proteins, Gene)
10.	Cytokines

Related Therapies and Procedures

1.	Therapeutics
2.	Drug Therapy (Chemotherapy)
3.	Highly Active Antiretroviral Therapy (HAART)
4.	Prostatectomy (Retropubic Prostatectomy)
5.	Bone Marrow Transplantation (Transplantation, Bone Marrow)