Paratuberculosis (Johne's Disease)

11/01/2006 - "paratuberculosis CF are strong candidates for use as antigens for improved serodiagnostic tests for bovine paratuberculosis."
01/01/2010 - "To further our understanding of this issue we studied the antigen-specific proliferation of lymphocytes, including lymphocyte subsets, during the course of early experimental ovine paratuberculosis and assessed differences in this response between animals that eventually succumbed to disease and those that remained disease-free. "
11/01/1985 - "Identification by enzyme-linked immuno-electrotransfer blot technique of M paratuberculosis antigens reactive in the ELISA will allow us to further study these antigens in the ELISA to improve sensitivity and specificity of the diagnostic test."
02/01/1947 - "paratuberculosis (Johne's bacillus); further studies on the nature of the antigen and on methods for demasking it."
06/01/1946 - "paratuberculosis (johne's bacillus); studies on the nature of the antigen and on methods for demasking it."

08/25/2006 - "Comparative efficacy of an IS900 tissue PCR, bacterial culture, johnin, agar-gel immunodiffusion (AGID) and absorbed-ELISA tests was investigated in 43 goats naturally infected with paratuberculosis. "
08/01/1997 - "To investigate the relation between lesions and serologic response in sheep with naturally acquired paratuberculosis and to evaluate the efficacy of agar gel immunodiffusion (AGID) and ELISA as diagnostic tests. "
07/01/2011 - "Parameters used herein to define paratuberculosis status were serum ELISA, serum agar gel immunodiffusion, cultureable fecal MAP, histopathology at necropsy and clinical disease. "
12/01/2010 - "avium paratuberculosis (MAP; n = 9), 0.125-2 mg/L for Mycobacterium kansasii (MKN;n = 2), 0.25-1 mg/L for Mycobacterium abscessus (MAB; n = 11), 4 mg/L for Mycobacterium smegmatis (MSMG; n = 1), and 1 and 8 mg/L for Mycobacterium ulcerans (MUL; n = 1), by microdilution and agar dilution methods, respectively. "
09/01/2007 - "A dairy herd, which had no imported animals, presented clinically suspect animals and was investigated for paratuberculosis using faecal culture, histopathology, indirect ELISA and the agar gel immunodiffusion test. "

09/01/2011 - "paratuberculosis subverts the immune response at the mucosal level in cattle is important for the development of improved disease control strategies, including new vaccines and diagnostic tests. "
01/01/2015 - "This information is required to develop improved diagnostics and effective vaccines for controlling Johne's disease. "
07/04/2003 - "Efficacy of paratuberculosis vaccines may be enhanced if calves are vaccinated prior to M. "
01/01/2014 - "Currently there is only one licensed vaccine within the United States and two vaccines licensed internationally against Johne's disease. "
02/01/2013 - "paratuberculosis vaccines in dairy herds: a mathematical modeling approach."

01/01/1975 - "In most of the cows investigated, the pathological changes of the ileocecal valve, characteristic for paratuberculosis, appeared earlier than the positive results of the complement fixation test."
04/01/1980 - "Bovine paratuberculosis I. A herd study using complement fixation and intradermal tests."
05/01/1961 - "Studies of Johne's Disease in Canada: X. A More Sensitive Complement-Fixation Test."
03/01/1960 - "Studies of Johne's Disease in Canada: VIII. Complement Fixation Tests with Protein Fractions of Johne's Bacilli."
11/01/1959 - "Studies Of Johne's Disease In Canada: IX. Comparison of Complement-Fixation Titres Prior To and At 24 or 48 Hours After A Johnin Intradermal Test."

01/01/1999 - "To investigate the relation between lesions and cellular immune response-based tests in sheep with naturally acquired paratuberculosis and to evaluate efficacy of an interferon-gamma (IFN-gamma) assay and the intradermal skin test. "
12/01/2011 - "paratuberculosis-specific gamma interferon (IFN-γ) in vaccinated calves by 7 days, with robust responses throughout the study. "
01/01/2011 - "Paratuberculosis with Murine Interferon Gamma in HeLa Cell Line and Study of Their Immunogenicity in Murine Model."
03/01/2006 - "The aim of the study reported here was to estimate the sensitivity and specificity of the gamma interferon (IFN-gamma) ELISA for paratuberculosis in sheep using receiver-operating characteristic analysis. "
10/15/2014 - "The interferon-gamma (IFN-γ) release assay is considered useful for diagnosis of subclinical paratuberculosis. "

08/01/2015 - "Although a previous study showed that feeding HT colostrum (60°C for 60min) produces short-term benefits, including improved passive transfer of IgG and reduced morbidity in the preweaning period, the current study found no benefit of feeding HT colostrum on long-term outcomes including risk for transmission of Mycobacterium avium ssp. paratuberculosis, milk production in the first and second lactation, and longevity within the herd. "
04/01/2015 - "The influence of milk yield and milk composition on the diagnosis of Mycobacterium avium ssp. paratuberculosis (MAP) by milk ELISA in the context of the total IgG secretion patterns in milk throughout lactation and serum concentrations were investigated. "
01/01/2012 - "The usefulness of IgG2 detection in serological tests for Johne's Disease diagnosis should be further evaluated."
01/01/2012 - "Detection of Bovine IgG Isotypes in a PPA-ELISA for Johne's Disease Diagnosis in Infected Herds."
12/01/2010 - "An ELISA to measure total IgG antibody specific against Mycobacterium avium ssp. paratuberculosis, the etiological agent that causes JD, and a quantitative real-time PCR to detect M. "

10/01/2006 - "Collectively, these results indicate that serologic tests for bovine paratuberculosis may be improved by using proteins derived from CF instead of CE. "
01/01/2007 - "paratuberculosis and will be useful for investigation of novel proteins, although SDS-PAGE/2D gel electrophoresis is recommended for study of high molecular weight species. "
03/01/1969 - "Studies of changes in rum proteins in cows and calves in a herd affected with Johne's disease."
11/07/2015 - "paratuberculosis proteins were identified by in silico analysis and need to be further evaluated for their immunodiagnostic and vaccine potential in field and mice model. "
07/01/2015 - "paratuberculosis proteins as potential targets for the host mitochondrial targeting. "

04/01/1950 - "Studies with the Johnin and tuberculin intradermal tests in cattle naturally infected with Mycobacterium paratuberculosis (Johne's disease)."
08/26/2010 - "Blood, serum and faeces were collected from 223 adult goats, from nine goat farms from Greece with a long-term record of paratuberculosis but no vaccination or tuberculin testing. "
06/01/2009 - "Interference of intradermal tuberculin tests on the serodiagnosis of paratuberculosis in cattle."
01/01/2009 - "paratuberculosis tuberculin purified protein derivative (PPD). "
09/01/2005 - "paratuberculosis, and purified protein derivative (PPD) from M. "

01/01/2011 - "Sera from fourteen calves immunized with LAM extract or PBS emulsified in Freund's Incomplete Adjuvant and from five paratuberculosis-infected bovines were studied. "
10/05/2004 - "Evaluation of a LAM ELISA for diagnosis of paratuberculosis in sheep and goats."
07/01/1994 - "The fecal samples were cultured for M paratuberculosis to identify infected cows, and the serum and milk samples were subjected to LAM ELISA testing. "
10/01/1991 - "After analytically controlling for management and cow factors in the respective regression models, positive serological paratuberculosis status (as defined by the LAM-ELISA test), was associated with higher milk somatic cell counts at both the herd average (p less than 0.01), and individual cow levels of organization (p less than 0.0001). "
10/01/1991 - "Associations between dairy production indices and lipoarabinomannan enzyme-immunoassay results for paratuberculosis."

01/01/2009 - "Monensin, called a "Growth Enhancer" in cattle, ameliorates Johne's disease without a documented mechanism of action. "
01/01/2009 - "We posit that the "Growth Enhancer" effect of Monensin may, at least in part, be due to inhibition of MAP in clinical or sub-clinical Johne's disease."
01/01/2009 - "Using the radiometric 14CO2 Bactec system, that expresses mycobacterial growth in arbitrary growth index (GI) units, we studied the effect of Monensin on the growth kinetic of MAP isolated from humans with IBD ("Dominic", "Ben" & UCF-4) and cattle with Johne's disease (303 & ATCC 19698.) Results are expressed as percent inhibition of cumulative GI (%-Delta cGI). "
10/17/2006 - "Monensin use might be associated with milk-ELISA positivity, but its impact on the transmission of paratuberculosis remains unknown."
10/17/2006 - "In the 46 herds with a prior history of paratuberculosis, feeding monensin to the breeding-age heifers was associated with decreased odds of a cow testing positive (OR=0.54). "

03/01/2001 - "paratuberculosis-specific polymerase chain reaction assays; (iii) development of a novel in situ hybridization method; (iv) efficacy of macrolide and anti-mycobacterial drug therapies; and (v) discovery of Crohn's disease-specific seroreactivity against two specific M. "
06/01/2001 - "paratuberculosis is a cause of Crohn's disease, widespread acceptance of this hypothesis will require evidence that specific anti-mycobacterial chemotherapy will cure the disease."

05/01/2010 - "paratuberculosis under in vitro culture conditions, has therapeutic value as a probiotic for adult cattle with paratuberculosis.  Animals were obtained from several herds with evidence of disease based on seropositivity and/or fecal shedding.  Sixty-eight cows with initial evidence of Stage II or III paratuberculosis and 2 with an initial Stage IV disease were evaluated longitudinally.  Animals were either treated daily with variable, disease-dependent doses of Dietzia (n = 48) or left untreated (n = 22).  Clinical aspects of disease (diarrhea, emaciated, cachectic and appetite) were recorded until the animal recovered or required euthanasia due to advanced clinical paratuberculosis or other severe conditions.  Paratuberculosis parameters-antibody serology (ELISA, AGID) and fecal culture-were longitudinally monitored over the lifetime of each animal.  The results indicated that daily treatment with Dietzia was therapeutic for paratuberculosis cows based on: (a) longitudinal decline in ELISA values only occurred in animals that were treated; (b) prolonged survival was dependant upon treatment--the length being directly associated with low initial ELISA values; and (c) treated animals were the only ones cured of disease.  Further investigations are envisaged to determine optimal, long-term dosages that may result in even better therapeutic outcomes as well as to evaluate potential application for therapy of the Johne's disease, human-counterpart, Crohn's disease."

07/01/2004 - "Paratuberculosis was experimentally produced in 20 crossbred lambs by oral administration of MAP to study the sequential development of lesions between 10 and 330 days postinfection and to assess commonly used diagnostic methods such as bacterial culture, lymphocyte stimulation test (LST), and enzyme-linked immunosorbent assay (ELISA) during progressive stages of infection. "
02/25/2005 - "Development of a Johne's disease infection model in laboratory rabbits following oral administration of Mycobacterium avium subspecies paratuberculosis."
12/01/1990 - "In this study we examined the effects of continuous oral administration of the immunosuppressive agents cyclophosphamide and prednisolone on the susceptibility of gnotobiotic nu/+ BALB/c mice to intestinal paratuberculosis. "

01/01/2014 - "paratuberculosis by using matrix-assisted laser desorption ionization-time of flight mass spectrometry."
12/01/2008 - "paratuberculosis, and 32 were identified by analysis of their tryptic peptide profile by matrix-assisted laser desorption ionization-time of flight analysis. "
01/01/2007 - "paratuberculosis: Comparison of methods for analysis by sodium dodecyl sulphate polyacrylamide gel electrophoresis, native PAGE and surface enhanced laser desorption/ionization time of flight mass spectrometry."

09/01/2012 - "Understanding this mechanism may contribute to the rational design of more effective vaccines and/or therapeutics for Johne's disease."
05/12/2009 - "paratuberculosis is critical to the development of effective vaccines and therapeutics for the control of this disease in the field. "