DNA Polymerase I (Klenow Fragment)

A DNA-dependent DNA polymerase characterized in prokaryotes and may be present in higher organisms. It has both 3'-5' and 5'-3' exonuclease activity, but cannot use native double-stranded DNA as template-primer. It is not inhibited by sulfhydryl reagents and is active in both DNA synthesis and repair.

Also Known As:

Networked: 239 relevant articles (4 outcomes, 18 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1.	Kuriyama, Isoko: 3 articles (01/2007 - 05/2005)
2.	Mizushina, Yoshiyuki: 3 articles (01/2007 - 05/2005)
3.	Yoshida, Hiromi: 3 articles (01/2007 - 05/2005)
4.	Banerjee, Sipra: 2 articles (01/2018 - 05/2006)
5.	Ma, Rui: 2 articles (01/2018 - 05/2006)
6.	Tuteja, Narendra: 2 articles (01/2018 - 05/2006)
7.	Maeda, Naoki: 2 articles (01/2007 - 10/2005)
8.	Sakaguchi, Kengo: 2 articles (01/2007 - 05/2005)
9.	Sugawara, Fumio: 2 articles (01/2007 - 05/2005)
10.	Hanaoka, Fumio: 2 articles (12/2005 - 04/2004)

Related Diseases

1.	Neoplasms (Cancer) 04/30/1995 - "Cancers with higher DNA polymerase alpha activity were associated with shorter disease-free and overall survival times. " 08/01/1999 - "The N-terminal 168 residues of RPA70 form a structurally distinct domain that stimulates DNA polymerase alpha activity, interacts with several transcriptional activators including tumor suppressor p53, and during the cell cycle it signals escape from the DNA damage induced G2/M checkpoint. " 08/01/1999 - "The average number of apoptotic cells was 19.9 (0 approximately 168)/5 HPF, and cases were classified into 3 groups based on the number of apoptotic cells/5HPF: 0 to 10, 11 to 30, and 31+. The level of apoptosis increased with increasing size of the tumor, and apoptosis was rarely seen in tumors with positive ER or lower proliferative activity, as assessed by DNA polymerase alpha. " 07/16/1999 - "Activities of Escherichia coli DNA polymerase-I were examined in the presence of the anti-tumor drug cis-diaminedichloroplatinum(II) and its inactive geometric isomer trans-diaminedichloroplatinum(II). " 07/16/1999 - "Inhibition of Escherichia coli DNA polymerase-I by the anti-cancer drug cis-diaminedichloroplatinum(II): what roles do polymerases play in cis-platin-induced cytotoxicity?"
2.	Neoplasm Metastasis (Metastasis) 03/01/1992 - "The results of multivariate analysis using the proportional hazard model of Cox indicated that the DNA polymerase alpha positive cell rates, liver metastasis, and peritoneal metastasis were independent prognostic factors. " 03/01/1992 - "There was no significant correlation between DNA polymerase alpha positive cell rates and histological type, tumour size, invasion of bowel wall, lymphatic invasion, venous invasion, lymph node metastasis or peritoneal metastasis. " 04/15/1999 - "Regarding the relation to clinicopathologic factors, a significant number of patients with estrogen receptor (ER) negative tumors who had a high level of DNA polymerase alpha, short disease free intervals (DFI), and metastases to other organs were included in Group C. "
3.	Burkitt Lymphoma (Burkitt's Lymphoma) 11/13/1997 - "Rb protein isolated from human Burkitt lymphoma Raji cells also stimulated DNA polymerase alpha. " 07/15/1978 - "DNA polymerase alpha + beta and DNA polymerase gamma activity were indiscriminately high in all immature cells as found in ALL, AML, Burkitt's lymphoma and phytohemagglutinin-stimulated normal lymphocytes, when compared to mature leukocytes found in normal individuals or in patients whose cancer was in remission. "
4.	Actinic Keratosis 09/15/2010 - "For an improved treatment of actinic keratosis and squamous cell carcinoma, inhibitors of human DNA polymerase alpha (pol alpha) are developed by docking nucleoside phosphonate diphosphates into the active site of pol alpha. "
5.	Squamous Cell Carcinoma (Epidermoid Carcinoma) 09/15/2010 - "For an improved treatment of actinic keratosis and squamous cell carcinoma, inhibitors of human DNA polymerase alpha (pol alpha) are developed by docking nucleoside phosphonate diphosphates into the active site of pol alpha. "

Related Drugs and Biologics

1.	Estrogen Receptors
2.	Nucleosides
3.	Diphosphates (Pyrophosphates)
4.	Phytohemagglutinins
5.	Organophosphonates (Phosphonates)
6.	DNA Polymerase gamma
7.	DNA (Deoxyribonucleic Acid)
8.	Bromodeoxyuridine (BrdU)
9.	Monoclonal Antibodies
10.	Enzymes

Related Therapies and Procedures

1.	Hepatectomy
2.	Drug Therapy (Chemotherapy)
3.	Aftercare (After-Treatment)