Dithiothreitol (Cleland's Reagent)

01/01/1990 - "Dithiothreitol was also found to be significantly protective against H2S poisoning. "
09/01/1986 - "In chronic lead exposure the enzyme was not invariably reactivated fully with dithiothreitol, indicating more severe enzyme poisoning. "
05/01/2010 - "The present study was undertaken to establish mode of action, comparative therapeutic efficacy and safety evaluation of N-acetyl cysteine and dithiothreitol against acute dimethylmercury poisoning in rats. "
11/01/1983 - "The authors studied the protective action of dithiols (unithiol, dithiothreitol) and their combinations with atropine in proserine poisoning. "
03/01/2008 - "Dimercaprol (BAL), a dithiol chelating agent therapeutically used for the treatment of heavy metals poisoning, was capable of protecting the [3H]-glutamate binding against the effects caused by Pb2+ and Hg2+. 2,3-Dimercaptopropane-1-sulfonic acid (DMPS), another dithiol-reducing chelating agent, was capable of protecting the effect caused by Cd2+, Pb2+ and Hg2+. The similar effect was observed with addition of dithiothreitol (DTT) on [3H]-glutamate binding in human platelets. "

04/15/1982 - "Protective effects of vitamin E and dithiothreitol against the hemolysis of rat and goat erythrocytes induced by Tween 20 with or without ascorbic acid and azide."
01/01/2008 - "According to flow-cytometry, Ca(2+) entry into human ghosts prepared by hemolysis in EGTA-buffered solution containing the Ca(2+) indicator Fluo3/AM was inhibited by the reducing agent dithiothreitol and the erythrocyte cation channel blockers ethylisopropylamiloride and amiloride. "
03/15/1984 - "Vitamin E, catalase, manganous or cobaltous ions and dithiothreitol protect against Tween 20-induced hemolysis of vitamin E-deficient chick and kid erythrocytes."
08/15/1980 - "In vivo or in vitro uptake of vitamin E by the erythrocytes, or the addition of dithiothreitol or 2-mercaptoethanol, prevented the hemolysis with Tween 20."
03/15/1984 - "Addition of catalase, MnCl2, CoCl2 or dithiothreitol in vitro showed significant protection against the hemolysis induced by Tween 20 in vitamin E-deficient chick and kid erythrocytes."

01/01/2014 - "We reveal that SSPEI-SPIO nanoparticles are capable of binding genes to form nano-complexes and mediating a facilitated gene release in the presence of dithiothreitol (5-20 mM), thereby leading to high transfection efficiency against different cancer cells. "
12/01/2012 - "Replication deficient Ad5 bearing wild-type fiber (Ad5wt) or RGD4G, RGD4C and RGD2C2G insertions within the HI loop of the fiber protein (Ad5RGD4G, Ad5RGD4C and Ad5RGD2C2G, respectively) were used to transduce a panel of cancer cell lines, with or without previous treatment of these Ad5s with the reducing agent dithiothreitol (DTT). "
01/01/1997 - "Dithiothreitol did not increase the basal activity of CB from SQCLC and the lung which indicates that reversibly oxidized forms of CB do not accumulate in the tumors and the lungs. "
08/01/1991 - "The antiproliferative effect was partially reversed by 10 microM of dithiothreitol suggesting that attack on thiol groups in cancer cells by highly reactive alpha, beta-unsaturated carbonyl moiety in the metabolites was responsible for the cytotoxic actions."
10/01/1988 - "For assay we used a low number of tumor cells per well and medium enriched with dithiothreitol-treated fetal calf serum. "

10/01/2001 - "In vitro excystation studies were carried out on the metacercariae cysts of Paragonimus heterotremus obtained from naturally infected crabs Potamon spp. The effects of elastase, trypsin, trypsin-dog bile, trypsin-bile salt, and dithiothreitol (DTT) were examined. "
01/01/1990 - "Preincubation of enzyme with sulfhydryl (-SH) agents such as cysteine (Cyst) and dithiothreitol (DTT) but not glutathione (GSH) protected against Pb-inhibition. "
12/01/1991 - "Sulfhydryl agents such as dithiothreitol (DTT) and cysteine (Cyst), but not glutathione (GSH) offered varied levels of protection against Pb-inhibition of Na(+)-K+ ATPase at pH 7.5 and 8.5. "
08/01/2009 - "Cyst walls were prepared by removing intracellular material from cysts by pre-treating them with sodium dodecyl sulphate (SDS) containing dithiothreitol, and then subjecting these to a series of sequential enzymatic digestions using amyloglucosidase, papain, DNase, RNase and proteinase K. "

08/01/1988 - "Protective efficacy of dithiothreitol in experimental acute renal failure: a verification with lipolytic activity of adipose tissue."
08/01/1977 - "The current study was undertaken to examine the effects of dithiothreitol (DDT), a sulfhydryl-reducing agent and heavy metal chelator, on the course of heavy metal-induced acute renal failure in the rat. "
09/30/1983 - "Amelioration of mercuric chloride-induced acute renal failure by dithiothreitol."
08/01/1977 - "Effect of dithiothreitol on mercuric chloride- and uranyl nitrate-induced acute renal failure in the rat."