Dihydroorotase

An enzyme that, in the course of pyrimidine biosynthesis, catalyzes ring closure by removal of water from N-carbamoylaspartate to yield dihydro-orotic acid. EC 3.5.2.3.

Also Known As:

Amidohydrolase, Dihydro-Orotate; Dehydrase, Carbamoylaspartic; Dihydro Orotase; Dihydro Orotate Amidohydrolase; Carbamoylaspartic Dehydrase; Dihydro-Orotase; Dihydro-Orotate Amidohydrolase; (S)-Dihydroorotate amidohydrolase

Networked: 22 relevant articles (0 outcomes, 2 trials/studies)

Bio-Agent Context: Research Results

Enzymes and Coenzymes: 1
- Enzymes: 152586
  - Hydrolases: 2166
    - Amidohydrolases: 43
      - Dihydroorotase: 22
        CAD trifunctional enzyme
        Ustilago maydis PYR3 protein

Experts

1.	Bubb, Larissa: 1 article (01/2020)
2.	Chen, Lin: 1 article (01/2020)
3.	Cheng, Mei-Ling: 1 article (01/2020)
4.	Chien, Kun-Yi: 1 article (01/2020)
5.	Delfarah, Alireza: 1 article (01/2020)
6.	Feng, Pinghui: 1 article (01/2020)
7.	Gao, Ruoyun: 1 article (01/2020)
8.	Graham, Nicholas A: 1 article (01/2020)
9.	Ho, Hung-Yao: 1 article (01/2020)
10.	Jiang, Taijiao: 1 article (01/2020)

Related Diseases

1.	Ascites 03/01/1964 - "STUDIES ON DIHYDROOROTASE ACTIVITY IN PREPARATIONS FROM NOVIKOFF ASCITES HEPATOMA CELLS." 04/25/1980 - "The effects of pH and inhibitors upon the catalytic activity of the dihydroorotase of multienzymatic protein pyr1-3 from mouse Ehrlich ascites carcinoma." 12/25/1979 - "Interconversion of carbamayl-L-aspartate and L-dihydroorotate by dihydroorotase from mouse Ehrlich ascites carcinoma." 07/01/1975 - "Purification of homogeneous glutamine-dependent carbamyl phosphate synthetase from ascites hepatoma cells as a complex with aspartate transcarbamylase and dihydroorotase." 06/10/1979 - "The inhibition of dihydro-orotase (E 3.5.2.3) and dihydroorotate (DHO) dehydrogenase (dihydro-orotate oxidase, EC 1.3.3.1) by cellular orotate (OA) in Ehrlich ascites cells was studied by measuring the accumulation of the intermediates of de novo pyrimidine biosynthesis at various times after the addition of 6-azauridine to the culture medium. "
2.	Neoplasms (Cancer) 11/19/2015 - "Here we show that decreased activity of ASS1 in cancers supports proliferation by facilitating pyrimidine synthesis via CAD (carbamoyl-phosphate synthase 2, aspartate transcarbamylase, and dihydroorotase complex) activation. " 01/01/2020 - "Here, we report that the enzyme carbamoyl-phosphate synthetase, aspartyl transcarbamoylase, and dihydroorotase (CAD) deamidates the RelA subunit of NF-κB in cancer cells to promote aerobic glycolysis and fuel cell proliferation in tumorigenesis. " 05/18/2023 - "In this study, the detection of genes associated with de novo uridine synthesis, carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, dihydroorotase (CAD) (n = 115), and dihydroorotate dehydrogenase (DHODH) (n = 115) in HCC tissues through tissue microarrays revealed that the expression of CAD and DHODH was higher in tumor compared with paraneoplastic tissues. " 11/20/2023 - "CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase and dihydroorotase) is a trifunctional enzyme that initiates the de novo pyrimidine synthesis, which is normally enhanced in cancer cells to preserve the pyrimidine pool for cell division. " 01/01/2019 - "Targeting the pyrimidine synthetic rate-limiting step enzyme carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, dihydroorotase (CAD) or the critical downstream enzyme dihydroorotate dehydrogenase (DHODH) inhibited GSC survival, self-renewal, and in vivo tumor initiation through the depletion of the pyrimidine nucleotide supply in rodent models. "
3.	Hepatocellular Carcinoma (Hepatoma) 03/01/1964 - "STUDIES ON DIHYDROOROTASE ACTIVITY IN PREPARATIONS FROM NOVIKOFF ASCITES HEPATOMA CELLS." 07/01/1975 - "Purification of homogeneous glutamine-dependent carbamyl phosphate synthetase from ascites hepatoma cells as a complex with aspartate transcarbamylase and dihydroorotase." 05/16/1978 - "Multi-enzyme complex of glutamine-dependent carbamoyl-phosphate synthetase with aspartate carbamoyltransferase and dihydroorotase from rat ascites-hepatoma cells. " 05/01/1981 - "Carbamoyl-phosphate synthetase II [EC 6.3.5.5] of rat ascites hepatoma cells (AH 13), the first and regulatory enzyme of de novo pyrimidine nucleotide biosynthesis, exists as a multienzyme complex (molecular weight, 870,000) with aspartate carbamoyltransferase [EC 2.1.3.2] and dihydroorotase [EC 3.5.2.3] (Mori, M. " 07/01/1975 - "Glutamine-dependent carbamyl phosphate synthetase [EC 2.7.2.9] was purified 1,300-fold from rat ascites hepatoma cells (AH 13) as a multienzyme complex with aspartate transcarbamylase[EC 2.1.3.2] and dihydroorotase[EC 3.5.2.3], using dimethyl sulfoxide, glycerol, and dithiothreitol as stabilizers. "
4.	Malaria 04/28/2002 - "5-Fluoroorotic acid (H(3)FOro) is a potent inhibitor for some metalloproteins such as dihydroorotase and dihydroorotate dehydrogenase and for thymidylate synthase (nonmetalloprotein) in the human malaria parasite Plasmodium falciparum. " 05/03/1994 - "The reaction, N-carbamyl-L-aspartate-->L-dihydroorotate (CA-asp-->DHO) catalyzed by malarial dihydroorotase is inhibited by L-6-thiodihydroorotate (TDHO) in vitro (Ki = 6.5 microM), and TDHO, as the free acid or methyl ester, induces a major accumulation of CA-asp in malaria. "
5.	Carcinoma (Carcinomatosis) 04/25/1980 - "The effects of pH and inhibitors upon the catalytic activity of the dihydroorotase of multienzymatic protein pyr1-3 from mouse Ehrlich ascites carcinoma." 12/25/1979 - "Interconversion of carbamayl-L-aspartate and L-dihydroorotate by dihydroorotase from mouse Ehrlich ascites carcinoma." 04/25/1980 - "We have studied factors affecting the catalytic activity of dihydroorotase (EC 3.5.2.3), purified as part of a multienzymatic protein which contains carbamyl phosphate synthetase, aspartate transcarbamylase, and dihydroorotase (ME pyr1-3) and which initiates de novo pyrimidine biosynthesis in mouse Ehrlich ascites carcinoma. "

Related Drugs and Biologics

1.	Ligases (Synthetase)
2.	Carbamyl Phosphate (Carbamoyl Phosphate)
3.	Aspartate Carbamoyltransferase
4.	Dihydroorotate Dehydrogenase
5.	Uridine
6.	pyrimidine
7.	Enzymes
8.	Aspartic Acid (Aspartate)
9.	Oxidoreductases (Dehydrogenase)
10.	4,5-dihydroorotic acid