Digitonin

A glycoside obtained from Digitalis purpurea; the aglycone is digitogenin which is bound to five sugars. Digitonin solubilizes lipids, especially in membranes and is used as a tool in cellular biochemistry, and reagent for precipitating cholesterol. It has no cardiac effects.

Also Known As:

Networked: 163 relevant articles (1 outcomes, 8 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1.	Wink, Michael: 5 articles (11/2015 - 10/2011)
2.	Eid, Safaa Yehia: 3 articles (12/2013 - 11/2012)
3.	El-Readi, Mahmoud Zaki: 3 articles (12/2013 - 11/2012)
4.	Esato, K: 2 articles (10/2001 - 01/2001)
5.	Kaneda, Y: 2 articles (10/2001 - 01/2001)
6.	Li, T S: 2 articles (10/2001 - 01/2001)
7.	Matsuoka, T: 2 articles (10/2001 - 01/2001)
8.	Tanaka, T: 2 articles (10/2001 - 01/2001)
9.	Zempo, N: 2 articles (10/2001 - 01/2001)
10.	Han, Yongsheng: 1 article (06/2022)

Related Diseases

1.	Autolysis 01/01/1975 - "4. Procedures such as treatment with digitonin, hypo-osmotic shock and acid autolysis, although effective in causing varying degrees of resolution of the latency of lysosome hydrolase activities, were unable to modify appreciably the pattern of dependence or independence of their fractional free activities on substrate concentration, as compared with that exhibited by control preparations. "
2.	Hemolysis 10/01/1984 - "Digitonin and its analogs showed activity to induce hemolysis, granulocyte activation and liposomal membrane damage. " 07/01/1980 - "Lowering of temperature slows down the rate of hemolysis and increases the quantity of digitonin required to obtain a fixed extent of hemolysis. " 07/01/1980 - "The third step (3) is hemolytic, and comprises the changes within the digitonin-altered membrane during hemolysis; it is stongly influenced by temperature. " 07/01/1980 - "The second step (2) is prelytic, related to the time required for bound digitonin molecules to alter the membrane structure so much that hemolysis may take place; this step has a transition temperature at 26 degrees C, probably related to the "melting" of specific membrane structures at that temperature. " 07/01/1980 - "The following minimal mechanism of hemolysis was postulated, based upon kinetic measurements: 2 D + E (1)in equilibrium E x D2 (2)leads to (E x D2) (3)leads to (E x D2) where D denotes a digitonin molecule and E a specific digitonin binding-site on the membrane. "
3.	Ehrlich Tumor Carcinoma 12/01/1984 - "The digitonin method for the study of cellular compartmentation in mitochondrial and cytosolic fractions was applied to Ehrlich ascites tumor cells. " 01/01/1989 - "Ehrlich ascites tumor cells were permeabilized using low concentrations of digitonin, 8 micrograms/10(6) cells. " 10/05/1988 - "(2) Evidence is reported that the addition of glucose to digitonin-permeabilized Ehrlich ascites tumor cells causes a decrease of mitochondrial membrane potential that coincided with a transient enhancement of the respiration rate and remained unchanged during the subsequent Crabtree effect. " 01/01/1984 - "1. Production of fluorescent formazan by unstimulated and phorbol ester- or digitonin-stimulated Ehrlich ascites tumor cells." 06/01/1995 - "To investigate the presence and the size of different non-mitochondrial Ca2+ pools of Ehrlich ascites tumor cells (EATCs), digitonin-permeabilized cells were allowed to accumulate Ca2+ in the presence of mitochondrial inhibitors and treated with the reticular Ca(2+)-ATPase inhibitor thapsigargin, IP3 and the Ca2+ ionophore A23187. "
4.	Pheochromocytoma 03/01/1999 - "This study tests for NPR-C effects on neurotransmitter release by examining fragments of the receptor for biological activity in pheochromocytoma (PC12) cells permeabilized with digitonin. " 06/01/2002 - "To investigate mAb influence on protein kinase regulation in live cells we permeabilized pheochromocytoma PC12 by digitonin. " 08/01/1995 - "We developed a convenient assay system for vesicular amine transport based on permeabilizing the plasma membrane of pheochromocytoma PC12 cells with digitonin at concentrations of 100 to 150 microM. " 08/15/1989 - "The inositol 1,4,5-trisphosphate (IP3)-sensitive Ca2+ compartment of endocrine cells was studied with alpha-toxin- and digitonin-permeabilized rat insulinoma (RINA2) and rat pheochromocytoma (PC12) cells. " 10/15/1985 - "The membrane-permeabilizing effects of streptolysin O, staphylococcal alpha-toxin, and digitonin on cultured rat pheochromocytoma cells were studied. "
5.	Lymphoma (Lymphomas) 10/10/1984 - "28Mg2+ influx studies in S49 murine lymphoma cells indicate that only 2-3% of total cell Mg2+ content can be exchanged at isotopic equilibrium, implying compartmentation of the newly transported Mg2+. The nature of this compartmentation was examined using selective permeabilization of the plasma membrane with the detergent, digitonin. " 10/15/1990 - "L1210 lymphoma cells were permeabilized with digitonin, and the ability of Ins(2,4,5)P3 and Ins(1,3,4,5)P4 to mobilize intracellular Ca2+ was studied. "

Related Drugs and Biologics

1.	Hydrolases
2.	Acids
3.	Detergents (Detergent)
4.	Proteins (Proteins, Gene)
5.	Saponins
6.	Enzymes
7.	Ions
8.	Glucose (Dextrose)
9.	Platinum
10.	Cisplatin (Platino)

Related Therapies and Procedures

1.	Pneumonectomy (Lung Volume Reduction)
2.	Intraperitoneal Injections
3.	Electrodes (Electrode)