Demecolcine

07/01/1993 - "The cell cycle analysis of high dose colcemid (2 micrograms/ml) treated Yac-1 cells showed an aneuploid or hypodiploid population. "
03/01/1993 - "A high proportion of oocytes activated following this treatment, but only the single-pronuclear haploid parthenogenones were incubated overnight in medium containing colcemid, to determine the incidence of aneuploidy as evidenced at first cleavage mitosis. "
09/01/1991 - "Using immunofluorescence to detect the presence of kinetochores in micronuclei, we were able to demonstrate a 26.8-fold increase in fluorescence-positive micronuclei (aneuploidy) in colcemid-treated cells. "
09/01/1987 - "The induction of aneuploidy in cultured mammalian cells has been studied using two chemical agents, diethylstilboestrol dipropionate and Colcemid, which act on the mitotic spindle, in order to investigate different methods of scoring and statistical analysis. "
01/01/1984 - "The dose response curves for colcemid induced morphological transformation and aneuploidy were similar. "

09/01/2001 - "Two cell lines (MDA886 and MSK922) and one primary tumor in short-term culture were subjected to metaphase growth arrest with colcemide in their exponential growth phase and fixed onto glass slides. "
01/01/2000 - "Thus, we established a convenient method to identify a target point in the cell cycle for anti-tumor agents using colcemid. "
01/01/2000 - "MI is reduced by anti-tumor agents used in conjunction with colcemid. "
09/15/1994 - "Cell cycle arrest by Colcemid differs in human normal and tumor cells."
08/01/1992 - "For this purpose we treated a tumor-derived cell line with Colcemid and analyzed the reversion of anchorage-independent phenotype in the subclones showing an altered association of the four marker chromosomes. "

09/26/1996 - "Comparison of spontaneous structural chromosome aberration frequency in 48 h-cultured human lymphocytes mitotically arrested by different colcemid treatments."
06/10/1996 - "For structural chromosomal aberration (SCA) analysis, cells were incubated for 18 h, and treated with Colcemid for 2 h before harvest. "
01/01/1996 - "The effect of Colcemid on the frequency and type of chromosomal abnormalities in human sperm was investigated. "
01/01/1996 - "Colcemid increases the frequency of chromosome abnormalities in human sperm."
03/01/1994 - "We further examined the effect of colcemid concentration on the quantitative consistency of the yields of radiation-induced chromosome aberration. "

06/15/1976 - "Data from this study of 24,455 mitotic spreads indicate increased concentrations of colcemid, as well as extended exposure times, results in a higher incidence of tetraploidy in cultured AFC, with no concomitant increase in the unexposed control cells."
05/01/2010 - "Pentaploid H1 (ES) cells (5H1 cells) were accidentally obtained through one-cell cloning of octaploid H1 (ES) cells (8H1 cells) that were established from tetraploid H1 (ES) cells (4H1 cells) polyploidized using demecolcine. "
11/01/2007 - "These characteristics of 4nH1K cells were similar to those of tetraploid 4nH1 cells that have been established through polyploidization by demecolcine, suggesting that 4nH1K and 4nH1 cells are similar irrespective of the different mechanisms of polyploidization."
06/01/2007 - "A tetraploid cell line (4nH1 cells) was established from mouse H-1 (ES) cells (2nH1 cells) highly polyploidized by treatment with demecolcine. "
06/01/2007 - "Establishment of a tetraploid cell line from mouse H-1 (ES) cells highly polyploidized with demecolcine."

09/01/1988 - "Fractionation and immunoblotting studies using rat hepatoma tissue culture cells blocked in metaphase with colcemid confirm the chromosomal localization of this 62-kD intranuclear protein during mitosis. "
02/01/1982 - "The intensity of cell proliferation in hepatoma was evaluated by the colcemide and autoradiography methods. "
10/15/1979 - "The specific activity and subcellular distribution of marker enzymes for the main subcellular components were analysed in homogenates of synchronized hepatoma cells (Morris 7288c), obtained by selective detachment at mitosis combined with a metaphase block with Colcemid. "
05/01/1974 - "Inhibition of transport systems in cultured rat hepatoma cells by colcemid and ethanol."
06/01/1985 - "Incubation of cells in colcemid, resulting in specific distribution of microtubules did not change either prekeratin or vimentin distribution in cells of both the hepatomas, but reversed polarization of elongated McA-RH-7777 cells. "