Autolysis

03/29/2013 - "With regard to their biochemical properties, the immobilized trypsin conjugates showed an increased resistance to elevated temperatures, eliminated autolysis, had an unchanged pH optimum and a significant storage stability and reusability. "
12/15/2010 - "The improved stability of EC-TR can be explained by covalent linkages on the surface of trypsin molecules, which effectively inhibits the denaturation, autolysis, and leaching of trypsin. "
11/25/1977 - "By using trypsin immobilized on glass beads, all possibilities of autolysis occurring during the denaturation process are eliminated. "
09/01/2008 - "The markers resulting from this autolysis were compared directly to those that evolved in a comparable 16 h on-tissue trypsin digest, and the markers that evolved in the two studies were seen to be substantially different. "
07/01/1966 - "[Study of enzymically active products of trypsin autolysis]."

04/01/1991 - "Meizothrombin autolysis could be described by apparent pseudo-first order kinetics and was not eliminated even in the presence of a 100-fold molar excess of DAPA. "
09/01/1982 - "Kinetic studies strongly suggested that the conversion of meizothrombin to meizothrombin-1 was due to ECV and not meizothrombin autolysis. "
11/09/2010 - "A remarkable feature of the structure is that the autolysis loop assumes a helical conformation enabling W148 and W215, located 17 Å apart in meizothrombin desF1, to come within 3.3 Å of each other and completely occlude access to the active site. "
12/15/1997 - "Meizothrombin rapidly loses the fragment 1 domain (F1) by autolysis to become meizothrombin des F1 (mzTBN-F1). "
04/01/1991 - "We conclude that the active site of human meizothrombin can never be completely blocked and autolysis can never be completely prevented even in the presence of saturating concentrations of the active site inhibitor DAPA."

01/01/2016 - "The best treatment condition was 750mgCOD/LSY+1200mgCOD/L ethanol giving higher COD RE, CE, CR (90±1%, 90±2% and 81±1% respectively), as compared with 1500mgCOD/LSY (76±2%, 63±7% and 48±4% respectively); ethanol addition was significantly favorable (p value=0.011), possibly due to electron availability and SY autolysis. "
12/01/2011 - "An extract of it, produced by autolysis, could replace yeast extract in culture medium with improved production of ethanol. "
05/01/2013 - "The effects of temperature, pH, and duration on the autolysis of an ethanol fermentation broth (EFB) were also investigated. "
04/01/2006 - "Considering the degree of autolysis, ethanol tolerance, and technical feasibility, we propose that deletion of the 3' end of the open reading frame of a single copy of BCY1 is a way to improve the quality of sparkling wines."
07/01/1995 - "[The effect of autolysis on characteristics of amino acid mixtures, obtained using ethanol-assimilating yeasts]."

01/17/1997 - "Interestingly, whereas oxidation did not inhibit autolytic conversion, the presence of high substrate concentrations did result in a significant reduction of autolysis without altering calpain proteolytic activity. "
12/01/2010 - "Our results suggest that the more rapid postmortem proteolysis found in BM than in LM at 5 °C similar with the previous study could be mainly explained by both greater amounts and faster activation and autolysis of µ-calpain in BM."
07/01/2005 - "Studies on the correlation between expression and/or autolysis of calpain and postmortem proteolysis in muscle have provided conflicting evidence regarding the possible role of calpain 3 in postmortem tenderization of meat. "
08/14/1998 - "A novel dissociation/autolysis mechanism for the activation of calpain at the membrane is proposed, which incorporates recent findings from structure-function studies of calpain, and its implications are discussed."
05/22/1998 - "In this study, the effects of oxidative stress on calpain-mediated proteolysis and calpain I autolysis in situ were examined. "

02/01/1995 - "Studies on the autolysis of m-calpain from the skeletal muscle of the amphibian Rana ridibunda."
07/01/2011 - "We propose a model for m-calpain activation that does not involve either autolysis or subunit dissociation."
07/01/2011 - "m-Calpain activation in vitro does not require autolysis or subunit dissociation."
11/01/2008 - "longissimus dorsi from three pigs collected at different times during storage further confirmed post-mortem autolysis of m-calpain. "
11/01/2008 - "The results indicated post-mortem autolysis of m-calpain as two m-calpain bands were observed on the zymogram gel. "

05/01/2015 - "The review presents data on the activity and some temperature characteristics of proteases in the potential food objects of fishes and some enteral microbiota representatives that provide induced autolysis and symbiotic digestion. "
04/07/2015 - "We report on an iconoclastic strategy for inhibiting proteases via autolysis acceleration. "
04/01/2015 - "In particular, deletion of MrSkn7 triggered cell autolysis with typical features such as cell vacuolization, downregulation of repressor genes, and upregulation of autolysis-related genes such as extracellular chitinases and proteases. "
02/01/2015 - "msaABCR operon positively regulates biofilm development by repressing proteases and autolysis in Staphylococcus aureus."
05/21/2014 - "Sea cucumber (Stichopus japonicus) autolysis during transportation and processing is a major problem and the specific proteinases responsible for autolysis have not yet been identified. "

04/01/2000 - "Previous studies in our laboratory have shown that the Staphylococcus aureus LytSR two-component regulatory system affects murein hydrolase activity and autolysis. "
07/01/2015 - "Newman ΔtagO showed enhanced susceptibility to autolysis, and an autolysin (atl) tagO double mutant abrogated this phenotype. "
04/01/2015 - "Consistent with reduced autolysis, the transcription levels of the autolysin genes, lytM and atlE, were reduced in the absence of Pfs. "
02/01/2015 - "The control of protease production mediates the processing of the major autolysin, Atl, and thus regulates the rate of autolysis. "
01/01/2015 - "To unravel the role of autolysis, an autolysin-coding gene, CA_C0554, was disrupted by using ClosTron system to obtain the mutant C. "

11/01/2011 - "Here we report how replacement of the flexible autolysis loop of human thrombin with the homologous rigid domain of the murine enzyme results in a drastic (up to 10-fold) increase in Na(+) affinity and a significant improvement in the catalytic activity of the enzyme. "
07/24/2009 - "Previous studies have shown that deletion of nine residues in the autolysis loop of thrombin produces a mutant with an anticoagulant propensity of potential clinical relevance, but the molecular origin of the effect has remained unresolved. "
01/01/2015 - "These results suggest that the peptide acts by binding to the active site as well as exosite I or the autolysis loop of thrombin. "
11/01/2011 - "Rigidification of the autolysis loop abolishes the heat capacity change associated with Na(+) binding observed in the wild-type and also increases the stability of thrombin. "
11/01/2011 - "Rigidification of the autolysis loop enhances Na(+) binding to thrombin."

12/05/1993 - "That all nine mAbs react with domain VI and not with the NH2-terminal domain V of the 28-kDa subunit suggests that domain VI (and not domain V) is involved in autolysis, contrary to the view that phosphatidylinositol lowers the Ca2+ concentration required for autolysis by binding to domain V."
06/15/1989 - "To test this hypothesis, we have compared the Ca2+ concentrations needed for autolysis and for proteolytic activity of the calpains both in the presence and the absence of phosphatidylinositol. "
06/15/1989 - "Phosphatidylinositol association lowers the Ca2+ concentration needed for autolysis, and autolysis, in turn, lowers the Ca2+ concentration needed for proteolytic activity. "
04/01/1986 - "Ca2+-Activated neutral protease (CANP), that consists of 80K and 30K subunits, is converted to a low-Ca2+-requiring form by autolysis in the presence of Ca2+. Phosphatidylinositol greatly reduces the Ca2+-requirement for the autolysis of native CANP. "
10/10/1984 - "Our results suggest that autolysis may be an important initial step in the activation of the Ca2+-dependent protease in vascular smooth muscle and that this step may be regulated by a combination of Ca2+ and phosphatidylinositol."

05/21/2009 - "The dextrin II-trypsin conjugate was more stable than the other conjugates and native trypsin at all temperatures between 30 and 70 degrees C, and also exhibited improved thermal stability in the autolysis assays at 40 degrees C."
05/21/2009 - "Dextrin-trypsin and ST-HPMA-trypsin conjugates: enzyme activity, autolysis and thermal stability."
05/21/2009 - "As we are currently developing dextrin- and semi-telechelic poly[N-(2-hydroxypropyl)methacrylamide] (ST-HPMA)-protein conjugates as new therapeutics, the aim of this study was to examine the effect of polymer on activity, autolysis and its thermal stability using trypsin conjugates as a model and compare to the data obtained for mPEG conjugates. "

06/01/2004 - "In addition to autolysis, the body's natural way of removing necrotic material, six methods of debridement are used in the UK today. "
08/01/1995 - "Debridement can be accomplished surgically (instrument/sharp), chemically, mechanically or by means of autolysis. "
06/01/2014 - "However, autolysis remains a frequently used method of debridement, despite recent recommendations from the European Wound Management Association and the National Institute for Health and Care Excellence. "
05/01/1996 - "(4) Results on time of 3.2 weeks to autolytic debridement of wounds with black eschar does not differ greatly from published time for enzymatic debridement, in spite of guidelines stating that autolysis may take longer than other methods. "
01/01/1999 - "Debridement can be accomplished surgically, mechanically (wet-to-dry gauze), or chemically (autolysis or topical enzymes). "

08/01/1996 - "Histological studies showed that there was less mucosal autolysis and sloughing of the grafts in the alpha-1-Pl group than in the other two groups. "
02/01/2006 - "Autolysis of one graft occurred at 4 weeks. "
05/01/2003 - "There was no gross evidence of graft autolysis. "
08/01/1996 - "The pathology of preserved and reperfused small intestinal grafts is characterized by mucosal autolysis and sloughing. "
01/01/1996 - "In 2 cases an autolysis of the graft occurred; one patient underwent prolonged corticosteroid therapy before, the other one was exposed extensively to chemical substances prior to surgery. "

09/01/2002 - "A full necropsy could not be performed because of autolysis of the tissues, but the surgical clips and the swim bladder appeared unremarkable. "
01/01/1997 - "We utilized antibodies against the proteinase inhibitors alpha-1-antichymotrypsin and alpha-2-macroglobulin, fibronectin and lysozyme to study samples of skin which had been intact intravitally, but were damaged postmortem either by autolysis or compression with a surgical clamp at the time of dissection. "

11/01/2006 - "This study presents fundamental biomechanical data on the mechanical response of the adult thorax that increases our understanding of any potential differences between PMHS and chest response obtained during CPR before substantial tissue autolysis begins. "
11/01/2006 - "In addition, the chest response during CPR demonstrated more hysteresis than the PMHS suggesting that the viscoelastic nature of the thorax is more pronounced when tissues are naturally perfused and substantial tissue autolysis has not begun. "

05/21/2009 - "As we are currently developing dextrin- and semi-telechelic poly[N-(2-hydroxypropyl)methacrylamide] (ST-HPMA)-protein conjugates as new therapeutics, the aim of this study was to examine the effect of polymer on activity, autolysis and its thermal stability using trypsin conjugates as a model and compare to the data obtained for mPEG conjugates. "