Ammonium Sulfate

03/01/2014 - "Hybridoma 4G5 was injected into the abdomen of Balb/c mice, and the anti-FB(1) mcAb was harvested from ascites and the titer reached 6.4 × 10(4) after purification with caprylic/ammonium sulfate precipitation (CA-AS) method. "
06/01/1993 - "Bmabs have been purified from mouse ascites by ammonium sulfate precipitation and affinity chromatography on immobilized peptide, modelling corresponding IL 2 epitope. "
02/15/1991 - "Ascites fluid was subjected to ammonium sulfate precipitation. "
09/15/1986 - "A single malignant ascites, when precipitated at 0 to 35% ammonium sulfate saturation, was the source of suppressive factor for purposes of isolation and standardization. "
11/01/1980 - "Meth A ascites cells were disrupted nuclei and membranes were removed by low- and high-speed centrifugation, and the soluble protein was fractionated by ammonium sulfate precipitation and gel filtration chromatography. "

01/01/1978 - "The extracts were separated into 50% ammonium sulfate soluble and insoluble fractions with the soluble fraction being more effective in blocking the cytotoxic responses through interaction with the lymphoid cells whereas the insoluble one had a greater effect upon tumor target cells. "
01/01/2006 - "Regarding carcinogenicity, ammonium sulfate did not exert any significant influence on the incidences of tumors in any of the organs and tissues examined. "
05/01/1989 - "Crude tumor extracts with 0.1 M Tris-HCl, pH 7.2 were fractionated by ammonium sulfate precipitation. "
01/01/1955 - "[Comparison of two method of purification of fowl tumor B77 virus by precipitation with ammonium sulfate and by fractional centrifugation]."
06/01/1986 - "Increased vascular permeability was inducible by the subcutaneous injection of a solid tumor extract rich in protein precipitable at 20-50% saturation of ammonium sulfate. "

09/01/1995 - "In this study, by using mouse MM46 mammary carcinoma as target, we purified the inhibitor from lysate of rat inflammatory peritoneal exudate cells by ammonium sulfate precipitation, gel filtration, isoelectrofocusing, and anion exchange chromatography. "
11/01/1978 - "Ascitic fluid from a patient with carcinoma of the pancreas was fractionated by ammonium sulfate precipitation. "
10/01/1972 - "I. Ammonium sulfate fractionation and its effect on the growth of Krebs-2 carcinoma."
03/15/1981 - "An ammonium sulfate fraction (0-25%) from an aqueous extract of pancreatic carcinoma tissues contains an antigen cross-reacting with extracts of other pancreatic carcinomas and various carcinomas representing all three germinal layers. "
08/01/1979 - "The effect of 60-F, a fraction obtained by 0.5 approximately 0.6 saturation of ammonium sulfate of streptomycin-pretreated cell-free extract from live hemolytic streptococci (avirulent Su strain), on release of 51Cr from the 51Cr-labeled Ehrlich ascites carcinoma cells was studied with following results: a) 60-F was found to be highly effective in releasing 51Cr from 51Cr-labeled Ehrlich ascites carcinoma cells. "

11/01/1994 - "Two major problems were encountered during the study: 1) the presence of a green-brownish pigment, which could not be eliminated by thermal shock or ammonium sulfate precipitation and 2) relative instability of enzymatic activity. "
01/01/1995 - "Fimbriae were removed from the bacterial cells by thermal shock at 60 C and then precipitated using ammonium sulfate. "
03/01/1994 - "Circulating shock protein was derived from plasma and was purified in two steps: stepwise ammonium sulfate precipitation followed by denaturing ion-exchange chromatography and refolding. "
06/22/2001 - "The release of FGF1 and S100A13 in response to heat shock results in the solubility of FGF1 at 100% (w/v) ammonium sulfate saturation, and the expression of a S100A13 deletion mutant lacking its novel basic residue-rich domain acts as a dominant negative effector of FGF1 release in vitro. "
01/01/1999 - "The coccoid form bacteria was treated with Ammonium Sulfate and heat shock before culture, and cultured in Brucella broth containing, sodium pyruvate, laked human erythrocyte and serum. "

07/01/1976 - "HeLa cell nuclei, isolated 17 h after infection with human adenovirus type 2 (Ad2), were treated with 200 mM ammonium sulfate. "
04/01/1988 - "Passive transfer of ammonium sulfate-precipitated 1073.10 ascites fluid into 2- to 3-day-old or 3-week-old chickens conferred protection against challenge infection with E. "
11/01/1977 - "When HeLa cell nuclei, isolated 17 h after infection with adenovirus type 2 (Ad2), were extracted with 200 mM ammonium sulfate, Ad2 nucleoprotein complexes were selectively released. "
08/01/1967 - "A lytic enzyme induced in Micrococcus lysodeikticus strain 1 by infection with N1 bacteriophage was purified 45- to 50-fold by ammonium sulfate precipitation, acid precipitation, and selective adsorption of contaminating proteins with calcium phosphate gel. "
03/01/1977 - "A procedure dependent on multiplicity of infection, incubation time, and treatment of crude lysates with diatomaceous earth was found to optimize phage yield, resulting in titers of 1 X 10(10) to 2 X 10(10) PFU/ml. Without prior treatment with diatomaceous earth, subsequent purification procedures (methanol, ammonium sulfate, polyethylene glycol) gave recoveries of less than 1% of crude lysate titers. "