cleavase

used for rapid 5'-3' endonucleolytic cleavage of the base of stem-loop structures formed when single stranded DNA is cooled without re-anealing; procedure is called cleavage fragment length polymorphism (CFLP)

Networked: 4 relevant articles (0 outcomes, 1 trials/studies)

Bio-Agent Context: Research Results

Experts

1.	Oh, Herin: 1 article (01/2011)
2.	Smith, Cassandra L: 1 article (01/2011)
3.	Amornrattana, A: 1 article (11/2000)
4.	Futrakul, A: 1 article (11/2000)
5.	Likidlilid, A: 1 article (11/2000)
6.	Ong-Ajyooth, S: 1 article (11/2000)
7.	Poldee, S: 1 article (11/2000)
8.	Pongrapeeporn, K U: 1 article (11/2000)
9.	Sutthikhum, V: 1 article (11/2000)
10.	Yamwong, P: 1 article (11/2000)

Related Diseases

1.	Hypercholesterolemia 11/01/2000 - "In this study DNA from 45 Thai subjects with primary hypercholesterolemia was screened for mutations in the hot spot exon 4. The DNA samples were amplified by Polymerase Chain Reaction (PCR) and screened for mutation by Cleavase Fragment Length Polymorphism (CFLP) technique. "
2.	Activated Protein C Resistance (APC Resistance) 01/01/2011 - "Protein-based detection assays include the conventional functional activated protein C resistance coagulation test and the recently reported antibody-mediated sensor detection; and DNA-based assays include approaches that use electrophoretic fractionation e.g., restriction fragment length polymorphism, denaturing gradient gel electrophoresis, and single-stranded conformational PCR analysis, DNA hybridization (e.g., microarrays), DNA polymerase-based assays, e.g., extension reactions, fluorescence polarization template-directed dye-terminator incorporation, PCR assays (e.g., amplification-refractory mutation system, melting curve analysis using real-time quantitative PCR, and helicase-dependent amplification), DNA sequencing (e.g., direct sequencing, pyrosequencing), cleavase-based Invader assay and ligase-based assays (e.g., oligonucleotide ligation assay and ligase-mediated rolling circle amplification). "
3.	Tuberculosis (Tuberculoses) 07/01/2000 - "Identification of drug-resistantMycobacterium tuberculosis by single strand conformation polymorphism and cleavase fragment length polymorphism."
4.	Long QT Syndrome 06/01/1998 - "Mutation Detection by Cleavase in Combination With Capillary Electrophoresis Analysis: Application to Mutations Causing Hypertrophic Cardiomyopathy and Long-QT Syndrome."
5.	Hypertrophic Cardiomyopathy (Hypertrophic Obstructive Cardiomyopathy) 06/01/1998 - "Mutation Detection by Cleavase in Combination With Capillary Electrophoresis Analysis: Application to Mutations Causing Hypertrophic Cardiomyopathy and Long-QT Syndrome."

Related Drugs and Biologics

1.	DNA (Deoxyribonucleic Acid)
2.	Proteins (Proteins, Gene)
3.	Oligonucleotides
4.	Ligases (Synthetase)
5.	Coloring Agents (Dyes)
6.	DNA-Directed DNA Polymerase (Polymerases, DNA)

Related Therapies and Procedures

1.	Ligation