Liberase

purified enzyme blend of collagenase isoforms I and II from Clostridium histoliticum and thermolysin from Bacillus thermoproteolyticus; used for isolation of islets of Langerhans from pancreas

Also Known As:

Networked: 15 relevant articles (1 outcomes, 1 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1.	Isachenko, Evgenia: 3 articles (12/2021 - 06/2018)
2.	Isachenko, Vladimir: 3 articles (12/2021 - 06/2018)
3.	Mallmann, Peter: 3 articles (12/2021 - 06/2018)
4.	Rahimi, Gohar: 3 articles (12/2021 - 06/2018)
5.	Hanstein, Bettina: 2 articles (10/2018 - 06/2018)
6.	Morgenstern, Bernd: 2 articles (10/2018 - 06/2018)
7.	Rappl, Gunter: 2 articles (10/2018 - 06/2018)
8.	Schmidt, Viola Maria: 2 articles (10/2018 - 06/2018)
9.	Carpéné, Christian: 2 articles (01/2018 - 09/2013)
10.	Itoh, T: 2 articles (12/2010 - 07/2010)

Related Diseases

1.	Exsanguination 05/01/2006 - "By multiple linear regression, the most significant variables that significantly improved islet yield were, firstly, the presence of <30 EU (endotoxin units) of endotoxin in Liberase batches, followed by a WIT under 10 min and the use of blood exsanguination before pancreas harvesting (P < 0.005). " 05/01/2006 - "Morphological screening of the pig pancreas before isolation, optimal blood exsanguination, WIT <10 min, and an endotoxin content <30 EU/mg in Liberase PI batches determine successful pig islet isolation for xenotransplantation in primates." 05/01/2006 - "The impact on islet yield of (1) pancreas procurement (blood exsanguination and warm ischaemia time (WIT)), (2) cold storage solutions (classic UW and modified UW (without hydroxyethyl starch and inverse K+/Na+ concentration)), (3) a dynamic or static method of pancreas digestion, and (4) the endotoxin content and enzymatic activity from five different batches of Liberase PI was studied. "
2.	Neoplasms (Cancer) 03/14/2023 - "Tumors injected multiple times with LQG and liberase also have lower tissue volumes but possess higher tissue moduli and lower viscoelasticities compared to the other treatment groups. " 03/14/2023 - "Examination of the Lissajous-Bowditch curves, Chebyshev coefficient ratios, elastic moduli, and dynamic viscosities demonstrate that the onset and type of nonlinear behavior is independent of treatment type and elastic modulus, suggesting that multiple liberase injections do not affect the nonlinear viscoelasticity of 4T1 tumors." 03/14/2023 - "We propose that a mechanotransductive mechanism could cause the formation of smaller but stiffer tumors after repeated, localized liberase injections. " 03/14/2023 - "Linear and nonlinear rheology of liberase-treated breast cancer tumors." 03/14/2023 - "Tumors that receive a single injection of localized liberase have significantly lower tumor volumes and lower tissue moduli at both the center and edge compared to buffer- and free liberase-injected control tumors, while tissue viscoelasticity remains relatively unaffected. "
3.	Overweight 01/15/2018 - "Adipocytes were prepared by liberase digestion from subcutaneous abdominal adipose tissue obtained from overweight subjects undergoing plastic surgery. " 09/01/2013 - "Samples of subcutaneous abdominal adipose tissue were obtained from overweight humans and immediately digested by liberase. "
4.	Prion Diseases (Transmissible Spongiform Encephalopathies) 12/01/2010 - "The potential for introducing transmissible spongiform encephalopathy (TSE) into islet cells was indicated by recognizing that Liberase HI is isolated from Clostridium histolyticum grown in media containing brain-heart infusion broth. " 08/15/2010 - "After the discontinuation of the manufacturing Liberase HI because of a small potential for prion disease transmission, Roche Diagnostics (Indianapolis, IN) developed a new enzyme product (Liberase MTF [mammalian tissue free]), which is similar to Liberase HI with the exception that no mammalian tissue is used in the manufacture of the collagenase component. "
5.	Necrosis 10/01/2018 - "It was concluded that the use of Tumor Dissociation Enzyme, effectiveness of which is higher than Liberase TM, does not lead to increasing of apoptosis and necrosis in follicles and stromal cells after enzymatic digesting of cryopreserved ovarian cortex." 01/01/2012 - "Islets isolated using Liberase HI showed higher viable β cells by NG/TMRE staining and decreased necrosis by Annexin V/DAPI staining. "

Related Drugs and Biologics

1.	Endotoxins
2.	Enzymes
3.	Collagenases (Collagenase)
4.	Thermolysin
5.	Liberase
6.	Solutions
7.	Dichlorodiphenyldichloroethane (DDD)
8.	Annexin A5
9.	Trypsin
10.	Starch (Cornstarch)

Related Therapies and Procedures

1.	Injections
2.	Heterologous Transplantation (Xenotransplantation)