Lowicryl 4KM resin

01/01/1989 - "The results obtained with our model system show that: the lesser chemical fixation, the more intense the labelling, and that dehydration by freeze-substitution preserves the antigenicity better than dehydration by progressive lowering of temperature, PLT, and finally that there is no obvious difference in labelling between the apolar Lowicryl HM20 and the polar Lowicryl K4M."
11/01/1986 - "We have modified the Lowicryl K4M low-temperature dehydration and embedding procedure for immunoelectron microscopy to provide improved ultrastructural detail and facilitate the localization of actin and tubulin in isolated rat adrenocortical cells, chick spinal cord with attached dorsal root ganglia (SC-DRG), and cultured dorsal root ganglia (DRG). "
05/01/1995 - "The present study compared Lowicryl K4M and Epon embedding after identical fixation and dehydration of rat somatotrophic secretory granules. "
02/01/1999 - "Samples from dry kidney bean cotyledons were fixed in acrolein vapor and embedded in Lowicryl K4M using a modified dehydration and embedding technique. "
01/01/1991 - "It can be partially protected by employing one of two strategies: processing at room temperature with partial dehydration (upto 70% solvent) and rapid embedding in LR White or Lowicryl K4M at 0 degrees C, or processing at progressively lower temperatures (PLT) and embedding in Lowicryl at -35/-50 degrees C. "

03/01/1995 - "To define precisely the intracellular localization of Leu-M1 in human adenocarcinomas, we have performed post-embedding immunoelectron microscopy with the protein A-gold technique on sections embedded in Lowicryl K4M from neoplasms of the lung, stomach, colon, and breast. "
02/01/1994 - "In this report, we have used postembedding protein A-gold immunocytochemistry on thin sections from tumor embedded in Lowicryl K4M to investigate the expression and ultrastructural localization of a panel of commercially available, diagnostically useful antibodies. "
12/01/1985 - "Second, using the postembedding approach, Lowicryl K4M sections of the tumor were exposed to antiserum against the amyloid P component and subjected to the protein A-gold procedure. "
12/01/1985 - "The presence of six substances--laminin, type IV collagen, heparan sulfate proteoglycan, entactin, fibronectin, and the amyloid P component--was investigated immunohistochemically in the matrix of the Engelbreth-Holm-Swarm (EHS) mouse tumor after it had been fixed in formaldehyde (with or without a brief preliminary glutaraldehyde fixation), embedded in Lowicryl K4M, and sectioned for processing through the protein A-gold sequence. "

01/01/1990 - "Cationic colloidal gold (CCG) and scrapie-infected mouse brain samples embedded in Lowicryl K4M were used for ultrastructural localization of negatively charged microdomains (anionic sites) in the cerebral microvasculature. "
01/01/1988 - "Lectin or glycoprotein-gold complexes and samples of scrapie-infected mouse brain embedded in Lowicryl K4M were used for ultrastructural localization of glycoconjugates. "

09/01/1986 - "The recently developed postembedding immunoelectron microscopic technique using low temperature embedding in Lowicryl K4M has been applied for the localization and relative quantitation of nephritogenic antibodies in rats with nephrotoxic serum nephritis. "
01/01/1990 - "Different types of antigens in normal rat and mouse kidneys as well as in tissues from cases of experimental induced nephritis were stained after Lowicryl K4M embedding by an immunogold (silver) method. "

03/01/1995 - "Previous work was carried out using post-embedding protein A-gold immunocytochemistry on thin sections embedded in Lowicryl K4M from a patient with Hodgkin's disease of the nodular sclerosing type; intense and precise labeling by gold particles was revealed in cytoplasmic granules, which were often clustered in a perinuclear location, in the Golgi apparatus, and focally along the plasma membrane of Reed-Sternberg cells. "
02/01/1994 - "To define the intracellular localization of Leu-M1 precisely, the authors performed postembedding immunoelectron microscopy with the protein A-gold technique on sections embedded in Lowicryl K4M from a patient with nodular-sclerosing-type Hodgkin's disease. "