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oligo(dT)-cellulose

Networked: 19 relevant articles (0 outcomes, 0 trials/studies)

Bio-Agent Context: Research Results

Related Diseases

1.	Infections 05/01/1976 - "Heterogeneous polyoma giant RNA molecules have been isolated by oligo(dT)- cellulose chromatography during the late phase of a lytic cycle of infection of mouse kidney cell cultures. " 03/01/1976 - "During late lytic infection of mouse kidney cell cultures polyoma 16S and 19S (late 19S RNA) were isolated by oligo(dT)-cellulose chromatography. " 09/01/1976 - "The procedure entails (i) the preparation of a crude cell lysate by Dounce homogenization of cells 3-5 h post-infection, (ii) differential centrifugation to give a 15 000 g 'mitochondrial' pellet, (iii) equilibrium centrifugation on discontinuous sucrose gradients (Friedman et al. 1972) to give a membranous band of density 1-16 g/ml, (iv) solubilization with Triton N-101 and velocity centrifugation to give a 25S solubilized polymerase complex and (v) affinity chromatography through an oligo (dT)-cellulose matrix bearing immobilized 42S virus particle RNA. " 03/01/1976 - "Early in lytic infection when viral DNA synthesis and the production of capsid protein are blocked by the addition of 5-fluorodeoxyuridine, approximately 100% of polyoma "early" 19S RNA was quantitatively retained by oligo(dT)-cellulose indicating the presence of poly(A) tracts on most 19S mRNA molecules. "
2.	Neoplasms (Cancer) 02/01/1986 - "Total poly(A+) RNA was prepared by phenol-chloroform-isoamyl alcohol extraction and oligo-dT-cellulose affinity chromatography from the tumor tissue and bovine parathyroid glands. " 05/01/1975 - "RNA extracted from these particles was chromatographed in oligo(dT)-cellulose solumns and the mRNA thus obtained characterized as collagen mRNA by its electrophoetical mobility in acrylamide gels (equivalent to 1.05 x 10-6 daltons) and its effect upon a cell-free system derived from Krebs ascites tumor cells. "
3.	Ascites 05/01/1975 - "RNA extracted from these particles was chromatographed in oligo(dT)-cellulose solumns and the mRNA thus obtained characterized as collagen mRNA by its electrophoetical mobility in acrylamide gels (equivalent to 1.05 x 10-6 daltons) and its effect upon a cell-free system derived from Krebs ascites tumor cells. " 11/21/1978 - "Ribonucleoprotein fragments were isolated from Ehrlich ascites cell messenger ribonucleoprotein (mRNP), immobilized on oligo(dT)-cellulose, by successive RNAase and formamide treatments and their RNA and protein moieties were characterized. "
4.	Sarcoma (Soft Tissue Sarcoma) 04/01/1973 - "Viral-specific RNA has been purified by oligo(dT)-cellulose chromatography from sarcoma virus-transformed nonproducer cells. " 04/01/1973 - "Purification by oligo(dT)-cellulose of viral-specific RNA from sarcoma virus-transformed mammalian nonproducer cells."
5.	Rabies (Hydrophobia) 07/01/1980 - "When labelled rabies-infected cell RNAs, which were purified by oligo(dT)-cellulose chromatography, were annealed to excess unlabelled virus RNA, digested with ribonuclease T2 and the RNA duplex molecules analysed by polyacrylamide gel electrophoresis, five duplexes could be separated. "

Related Drugs and Biologics

1.	Messenger RNA (mRNA)
2.	RNA (Ribonucleic Acid)
3.	Sucrose (Saccharose)
4.	Proteins (Proteins, Gene)
5.	Poly A
6.	Chloroform (Trichloromethane)
7.	guanidine thiocyanate (guanidinium thiocyanate)
8.	messenger ribonucleoprotein
9.	polyacrylamide
10.	Capsid Proteins (Capsid Protein)