Bacillus amyloliquefaciens ribonuclease

10/01/2014 - "The expression of the cytotoxin Barnase controlled by these promoters generated pollen specific ablation and male sterility. "
06/24/2014 - "We describe a hybrid wheat system that relies on the expression of a phytotoxic barnase and provides for male sterility. "
02/01/2014 - "The PR10gPro::barnase transgenic lines exhibited complete male sterility because of the disruption of the tapetum and the deformation of microspore/pollen. "
12/01/2013 - "Furthermore, the average barnase copy number in the plants displaying male sterility could be reduced. "
12/01/2013 - "The barnase fragments were assembled into a functional cytotoxin via intein-mediated trans-splicing, thus leading to male sterility through pollen ablation. "

01/01/2008 - "These results demonstrate that barnase is a potent toxic agent for targeting to cancer cells."
01/01/2008 - "Barnase as a new therapeutic agent triggering apoptosis in human cancer cells."
05/01/2007 - "Visualization of cancer cells by means of the fluorescent EGFP-barnase protein."
01/01/1996 - "Further, since domain Ia can be replaced with other cell targeting moieties, we propose that barnase-toxins should be evaluated for utility in targeted cancer therapy."
02/01/2011 - "Barnase, a ribonuclease from Bacillus amyloliquefaciens, is a promising candidate for targeted delivery to cancer cells because of its insusceptibility to the ubiquitous cytoplasmic ribonuclease inhibitor, and its high stability and catalytic activity. "

02/01/2008 - "The AG-I-35S (-60)::Barnase construct can be useful in diminishing pollen and seed formation in plants, providing a novel bisexual sterility strategy for interception of transgene escape and has other potentially commercial use for transgenic engineering."
02/01/2008 - "To explore a new approach to generating reproductive sterility in transgenic plants, the barnase gene from Bacillus amyloliquefaciens was placed under the control of an 1853-bp nucleotide sequence from the 3'end of the second intron of Arabidopsis AGAMOUS and CaMV 35S (-60) minimal promoter [AG-I-35S (-60)::Barnase], and was introduced into tobacco through transformation mediated by Agrobacterium tumefaciens. "
12/01/2006 - "To establish a simple and an efficient system to minimize the environmental risk of genetically modified plants, we tested the applicability of the barnase/barstar system in conferring bisexual sterility; that is, in preventing plants setting seeds by self-fertilization and out-crossing. "
12/01/2006 - "Bisexual sterility conferred by the differential expression of barnase and barstar: a simple and efficient method of transgene containment."
05/01/2008 - "To test their utility for engineering sterility, the promoters were fused to the Diphtheria toxin A (DT-A) gene coding for a ribosome inactivating protein as well as the Barnase gene coding for an extracellular ribonuclease, and tested for tissue-specific ablation. "

04/01/2005 - "We used a barstar gene driven by a heat shock or 35S promoter as a RC, and two different promoters were used for barnase genes in the BC. "
01/01/2011 - "Systematic analysis on different proteins reveals that the proteins azurin, cold shock protein, and C-terminal domain of ribosomal protein L9 are influenced by the number of medium- and long-range proteins, whereas barnase, FK506 binding protein, and IM9 are influenced by surrounding residues. "
03/21/2008 - "We investigate the average inter-residue folding forces derived from mutational data of the 15 proteins: barstar, barnase, chymotrypsin inhibitor 2 (CI2), Src SH3 domain, spectrin R16 domain, Arc repressor, apo-azurin, cold shock protein B (cspB), C-terminal domain of ribosomal protein L9 (CTL9), FKBP12, alpha-lactalbumin, colicin E7 immunity protein 7 (IM7), colicin E9 immunity protein 9 (IM9), spectrin R17 domain, and ubiquitin. "

12/01/2010 - "The betasatellite/split intact barnase gene cassette ensures that there is no expression of the barnase in the absence of TLCV-Au, but upon infection of the cell with the virus, release of the betasatellite/split barnase cassette as a replicating molecule resulting in the reconstitution and expression of an active barnase gene and the destruction of the infected cell. "
12/01/2010 - "Approximately one third of the transgenic lines containing the betasatellite/split barnase gene constructs were shown to be completely resistant to the TLCV-Au infection. "
10/01/2006 - "These results suggest that barnase-encoding vectors should be useful for negative selection strategies examining retroviral infection from entry to integration. "
10/01/2006 - "To identify cellular genes required for infection we used barnase-encoding vectors in a genetic screen to isolate mutant mammalian cells that are resistant to infection by MLV and HIV-1. "
10/01/1998 - "Cytotoxinic effect caused by expression of barnase was observed in four mammalian cells after vAcCMVBar infection Dose-dependent analysis revealed that one of the cells HICAM was the most sensitive to the virus infection. "