quinolinium dibromide (QdB)

01/01/2023 - "ELISA-like QDB method to meet the emerging need of Her2 assessment for breast cancer patients."
01/01/2020 - "Developing a routine lab test for absolute quantification of HER2 in FFPE breast cancer tissues using Quantitative Dot Blot (QDB) method."
01/01/2021 - "Methods: In this descriptive observational retrospective study, ER, PR, Her2, and Ki67 protein levels were measured absolutely and quantitatively in 852 FFPE breast cancer tissues using the QDB method. "
01/01/2021 - "Improving Prognosis of Surrogate Assay for Breast Cancer Patients by Absolute Quantitation of Ki67 Protein Levels Using Quantitative Dot Blot (QDB) Method."
01/01/2020 - "We were able to measure HER2 protein levels using 0.5 µg/sample total protein lysate extracted from 2 × 5 µm FFPE slices absolutely and quantitatively using QDB method in 332 breast cancer FFPE samples. "

01/01/2021 - "Our study also demonstrated that the QDB method is an effective tool to analyze archived FFPE cancer specimens in biomedical research."
01/01/2023 - "Our results support further evaluation of the QDB method as an alternative to IHC to meet the emerging need of identifying tumors with low Her2 expression (Her2-low) in daily clinical practice."
01/01/2023 - "Using the limit of detection (LOD) of the QDB method as the putative cutoff point, tumors with no Her2 expression were identified with the number comparable to those of IHC 0. "
01/01/1979 - "The binding characteristics of the cancer chemotherapeutic Cain's quinolinium 6-amino-1-ethyl-4-[p-[[p-[(1-ethylpyridinium-4-yl) amino] phenyl] carbamoyl]-anilino]-quinolinium dibromide (NSC 113089) to lipid extracts from rat kidney, liver, heart and skeletal muscle has been studied. "

01/01/2021 - "In this study, a quantitative, objective, absolute, and high throughput immunoblot method, quantitative dot blot (QDB), was explored to address this issue by investigating the putative relationship between estrogen receptor (ER)/progesterone receptor (PR) and human epidermal growth factor receptor 2 (Her2) pathways in breast cancer tumorigenesis. "

10/01/1981 - "Cain's Quinolinium; quinolinium, 6-amino-1-methyl-4[[[[[[(1-methyl-pyridinium-4-yl)amino]phenyl]amino]carbonyl]phenyl]amino] (NSC 176319), is a chemotherapeutic agent, which is equally cytotoxic in vitro to both murine L1210 leukemia cells and bone marrow progenitor cells. "
01/01/1978 - "The disposition of quinolinium dibromide (QdB), a highly active agent against murine L1210 leukemia was studied using 14C-labeled drug. "
10/01/1981 - "Cain's quinolinium (NSC 176319): protection of murine L1210 leukemia cells and bone marrow progenitor cells against mechlorethamine cytotoxicity and its application to combination chemotherapy."

12/01/2021 - "A QDB-based assay was developed for gastric cancer specimens to measure EGFR protein levels absolutely, quantitatively, and objectively. "
12/01/2021 - "Slices of 2×5 μm from formalin-fixed paraffin-embedded gastric cancer specimens were used to extract total tissue lysates for QDB measurement. "