cibacron blue-sepharose

affinity chromatography of alpha-2-macroglobulin

Also Known As:

Blue Sepharose CL-6B; Cibacron Blue Sepharose; cibacron blue F 3G-A sepharose; cibacron blue F3GA-agarose; cibacron blue agarose; cibracron blue-sepharose

Networked: 11 relevant articles (0 outcomes, 0 trials/studies)

Bio-Agent Context: Research Results

Carbohydrates: 22023
- Polysaccharides: 15383
  - Sepharose: 2476
    - cibacron blue-sepharose: 11

Related Diseases

1.	Experimental Liver Neoplasms 01/01/1993 - "Comparison of pyruvate kinase variants from rat liver and Morris hepatoma 7777, obtained by an affinity chromatography on blue sepharose CL-6B." 01/01/1993 - "Fractions A (salted out by ammonium sulphate between 21-30% saturation), and fractions B (salted out between 51-70% saturation) of pyruvate kinase (EC 2.7.1.40.) corresponding respectively to pyruvate kinase types L and M2 from rat liver and Morris hepatoma 7777 were purified by an affinity chromatography on Blue Sepharose CL-6B. " 01/01/1998 - "Cytosolic fractions B (salted out between 51-70% ammonium sulphate saturation) from rat liver and Morris hepatoma 7777, containing pyruvate kinase (EC 2.7.1.40) M2 isoenzymes, were purified by affinity chromatography on Blue Sepharose CL-6B. " 01/04/1996 - "Cytosolic pyruvate kinase fractions A and B obtained by salting out procedure from normal rat liver and Morris hepatoma 7777, purified by affinity chromatography on Blue Sepharose CL-6B, have shown similar electrophoretic patterns in polyacrylamide gel at pH 8.3 to previously studied pyruvate kinase extracts from chromatin of cell nuclei. "
2.	Neoplasms (Cancer) 07/01/1980 - "Different chromatographic behaviours on blue sepharose CL-6B of free and estradiol complexed forms of the estrogen receptor from DMBA-induced rat mammary tumors." 06/06/1988 - "In order to analyse the incorporation of phosphate in the respective isozymes after endogenous phosphorylation of cytosolic extracts with [32P]ATP, homotetrameric isozymes as well as heterotetrameric hybrids of differentiated tumors were resolved by affinity chromatography on Blue-Sepharose CL-6B and, if necessary, further purified by immunoprecipitation. "
3.	Small Cell Lung Carcinoma (Small Cell Lung Cancer) 08/01/1992 - "An immunotoxin (IT) comprising abrin A chain attached to the mouse monoclonal antibody SWA11, recognising a cell surface antigen highly associated with human small cell lung cancer (SCLC), was synthesised using a hindered disulphide crosslinker, N-succinimidyl 3-(2-pyridyldithio) butyrate (SPDB), and purified by Blue Sepharose CL-6B affinity chromatography. "
4.	Duchenne Muscular Dystrophy (Muscular Dystrophy, Becker) 01/01/1987 - "Two isozymes of adenylate kinase from human Duchenne muscular dystrophy serum, one of which was an aberrant form specific to DMD patients, were separated by Blue Sepharose CL-6B affinity chromatography. "
5.	Seminoma 02/01/1987 - "An alkaline phosphatase (EC 3.1.3.1) of the placental type was isolated from a seminoma type of human testicular cancer tissue and was purified to homogeneity by sulfate-mediated chromatography on a column of Cibacron Blue Sepharose 4B. "

Related Drugs and Biologics

1.	Pyruvate Kinase
2.	Isoenzymes
3.	Ammonium Compounds
4.	Octoxynol (Triton X 100)
5.	Sulfates (Sulfates, Inorganic)
6.	Salts
7.	Estrogen Receptors
8.	Poly I
9.	Poly C
10.	Phosphates (Orthophosphate)