| Abstract | Phospholipase D (PLD) neutralization was used to examine sera of humans (n = 40) with a spontaneous infection by Arcanobacterium haemolyticum, sheep and goats (n = 76 and 79 respectively) with a spontaneous infection by Corynebacterium pseudotuberculosis, mice (n = 26) experimentally immunized with PLD from A. haemolyticum (PLD-A) and mice (n = 28) experimentally immunized with PLD from C. pseudotuberculosis (PLD-C). PLD-A and PLD-C were also used as neutralizing antigens. A positive result of neutralization was due to an inhibition of the haemolytic synergism with the equi factor from Rhodococcus equi. The titres of sera neutralizing the homologous PLD were always significantly higher than those neutralizing the heterologous PLD. The proportion of sera that were able to neutralize the homologous PLD in sheep, goats and mice immunized with PLD-A significantly exceeded the proportion of sera that neutralized the heterologous PLD. The antigenic properties of PLD-A and PLD-C were similar but not identical. |
| Authors | B Skalka, I Literák, P Chalupa, M Votava
(Affiliation: Department of Microbiology, Masaryk University Medical School, Brno, Czech Republic.)
|
| Journal | Zentralblatt für Bakteriologie : international journal of medical microbiology
(Zentralbl Bakteriol)
Vol. 288
Issue 4
Pg. 463-70
(Dec 1998)
ISSN: 0934-8840 GERMANY |
| PMID | 9987184
(Publication Type: Journal Article)
|
| Chemical References |
- Antibodies, Bacterial
- Phospholipase D
|
| Topics |
- Actinomycetales
(enzymology, immunology)
- Actinomycetales Infections
(diagnosis, microbiology)
- Animals
- Antibodies, Bacterial
(blood)
- Corynebacterium Infections
(diagnosis, microbiology, veterinary)
- Corynebacterium pseudotuberculosis
(enzymology, immunology)
- Goat Diseases
(diagnosis, microbiology)
- Goats
- Humans
- Mice
- Neutralization Tests
- Phospholipase D
(immunology, metabolism)
- Serologic Tests
- Sheep
- Sheep Diseases
(diagnosis, microbiology)
|
