Protective effect of anisodamine on reperfusion injury of skeletal muscles in rabbit.

Abstract

Anisodamine is an alkaloid isolated from a Chinese plant, which was subsequently synthesized. Its chemical structure is similar to atropine. It inhibits cholinergic nerve function, improves microcirculation, and was reported to have a protective effect on reperfusion injury in various organs. We used anisodamine in a rabbit model with ischemia and reperfusion injury of hind limb muscles. We evaluated its effect on skeletal muscle cells, using transmission electron microscopy, and analyzed lipid peroxidation by measuring malondialdehyde and lactate dehydrogenase blood concentrations. We found that malondialdehyde and lactate dehydrogenase concentrations after 1 hour of reperfusion were lower in animals treated with anisodamine than in controls. Damage to membrane structures and myofilaments in muscle cells was less severe after anisodamine treatment. Our findings indicate that anisodamine protects skeletal muscles with ischemia and reperfusion injury.

• Authors: X Wei, Z Zhang, L Han, Y Wei
• Journal: Acta orthopaedica Scandinavica (Acta Orthop Scand) Vol. 69 Issue 6 Pg. 633-7 (Dec 1998) ISSN: 0001-6470 [Print] England
• PMID: 9930112 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Free Radical Scavengers
• Solanaceous Alkaloids
• anisodamine
• Malondialdehyde
• L-Lactate Dehydrogenase

Topics

• Animals
• Free Radical Scavengers (pharmacology)
• L-Lactate Dehydrogenase (blood)
• Lipid Peroxidation (drug effects)
• Malondialdehyde (blood)
• Microscopy, Electron
• Muscle, Skeletal (blood supply, pathology)
• Rabbits
• Reperfusion Injury (pathology)
• Solanaceous Alkaloids (pharmacology)