The identification and differential expression of calcium-binding proteins associated with ocular melanoma.

Abstract

Calcium-binding proteins may endow tumor cells with properties related to their malignancy and metastatic phenotype. Chromatographic procedures and amino acid sequence analysis were used in this study to identify seven calcium-binding proteins, annexin VI, cap g, annexin V, calmodulin, S100A11, S100B and S100A6, associated with uveal melanoma, the primary ocular tumor of adults. This series of calcium-binding proteins was identified in both primary tumors and cell lines of uveal melanoma. Several of the proteins were shown by immunochemical methods to be differentially expressed between normal uveal melanocytes and malignant melanomas of the uvea. In addition, the expression of S100A6 may correlate with the malignant properties of the tumor.

• Authors: P R Van Ginkel, R L Gee, T M Walker, D N Hu, C W Heizmann, A S Polans
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1448 Issue 2 Pg. 290-7 (Dec 10 1998) ISSN: 0006-3002 [Print] Netherlands
• PMID: 9920419 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Annexin A6
• Antibodies
• Microfilament Proteins
• Nerve Growth Factors
• Nuclear Proteins
• S100 Calcium Binding Protein beta Subunit
• S100 Proteins
• S100B protein, human
• S100A11 protein, human
• CAPG protein, human

Topics

• Adult
• Annexin A6 (immunology)
• Antibodies (immunology)
• Humans
• Melanoma (metabolism)
• Microfilament Proteins (analysis)
• Nerve Growth Factors (analysis)
• Nuclear Proteins (analysis)
• S100 Calcium Binding Protein beta Subunit
• S100 Proteins (analysis, biosynthesis, immunology)
• Tumor Cells, Cultured
• Uveal Neoplasms (metabolism)