Abstract | OBJECTIVE:
Actarit (4-acetylaminophenylacetic acid), developed in Japan, has been shown to be effective for suppressing disease activity of rheumatoid arthritis (RA). We analyzed effects of actarit on synovial cell functions in patients with RA for insight into the clinical application of this medication. METHODS: RA primary synovial cells were co-cultured with actarit at 10(-4)-10(-7) M. Their subsequent proliferative responses and proinflammatory cytokine and matrix metalloproteinase ( MMP) production at the mRNA and protein levels were measured. Effects of actarit on adhesion molecule expression were analyzed by immunofluorescence flow cytometry and cell-cell binding assay. RESULTS: CONCLUSION: The results suggest that actarit acts on RA synovial cells to reduce cell-cell interactions with autologous synovium infiltrating lymphocytes and to inhibit proinflammatory cytokine and MMP production, leading to amelioration of symptoms of RA.
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Authors | Y Takeba, N Suzuki, S Wakisaka, H Nagafuchi, S Mihara, A Kaneko, T Asai, T Sakane |
Journal | The Journal of rheumatology
(J Rheumatol)
Vol. 26
Issue 1
Pg. 25-33
(Jan 1999)
ISSN: 0315-162X [Print] Canada |
PMID | 9918236
(Publication Type: Journal Article)
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Chemical References |
- Antirheumatic Agents
- Cell Adhesion Molecules
- Cytokines
- Isoenzymes
- Membrane Proteins
- Phenylacetates
- RNA, Messenger
- 4-(acetylamino)benzeneacetic acid
- Cyclooxygenase 2
- PTGS2 protein, human
- Prostaglandin-Endoperoxide Synthases
- Collagenases
- Matrix Metalloproteinase 1
- Dinoprostone
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Topics |
- Antirheumatic Agents
(pharmacology)
- Arthritis, Rheumatoid
(drug therapy, enzymology, pathology)
- Cell Adhesion
(drug effects)
- Cell Adhesion Molecules
(biosynthesis)
- Collagenases
(biosynthesis, genetics)
- Cyclooxygenase 2
- Cytokines
(biosynthesis)
- Dinoprostone
(biosynthesis)
- Female
- Fibroblasts
(drug effects, metabolism)
- Gene Expression
(drug effects)
- Humans
- In Vitro Techniques
- Isoenzymes
(biosynthesis)
- Lymphocytes
(drug effects, metabolism)
- Male
- Matrix Metalloproteinase 1
- Membrane Proteins
- Middle Aged
- Phenylacetates
(pharmacology)
- Prostaglandin-Endoperoxide Synthases
(biosynthesis)
- RNA, Messenger
(metabolism)
- Synovial Membrane
(cytology, drug effects, metabolism)
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