Killing effects of 5-fluorouracil on human biliary tract cancer cell lines.

Abstract

Data concerning the cellular sensitivity of human biliary tract cancer cell lines to 5-FU are scarce. The purpose of the present study was to evaluate the cellular sensitivity of Mz-ChA-2 (derived from gall bladder cancer) and SK-ChA-1 (derived from bile duct cancer) to 5-FU. The clonogenic capacity of these lines after pulse (1 h) or continuous (168 h) administration of various 5-FU concentrations (0.1-100 microg/ml) was evaluated in exponentially growing cells as well as in those that had reached the plateau phase. In both cell lines, exponentially growing cells were 1.8-times more susceptible to 5-FU than those in plateau phase. However, when cells in the same growth phase were compared, Mz-ChA-2 cells were 10 times more sensitive to the drug than SK-ChA-1 cells. Regardless of the growth phase or cell line, increasing the duration of exposure to 5-FU decreased the proportion of surviving cells. Even at a non-cytocidal dosage, exposing the cells for three doubling times markedly decreased the number of viable cells remaining after treatment.

• Authors: Y Moon, T Todoroki, T Ohno, K Fukao, J B Little
• Journal: International journal of oncology (Int J Oncol) Vol. 14 Issue 2 Pg. 253-7 (Feb 1999) ISSN: 1019-6439 [Print] Greece
• PMID: 9917499 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antimetabolites, Antineoplastic
• Fluorouracil

Topics

• Antimetabolites, Antineoplastic (pharmacology, therapeutic use)
• Biliary Tract Neoplasms (drug therapy, pathology)
• Cell Death (drug effects)
• Cell Division (drug effects)
• Dose-Response Relationship, Drug
• Fluorouracil (pharmacology, therapeutic use)
• Humans
• Tumor Cells, Cultured