Treatment of brain edema with a nonpeptide arginine vasopressin V1 receptor antagonist OPC-21268 in rats.

Abstract

OBJECTIVE:

Recent experimental evidence suggests that centrally released arginine vasopressin plays a significant role in brain capillary water permeability as well as in pathogenesis of vasogenic brain edema. The purpose of this study was to examine the effects of orally administered OPC-21268, a nonpeptide arginine vasopressin V1 receptor antagonist, on cold-induced brain edema in rats.

METHODS:

Cold brain injury was induced for 1 minute in 140 rats. Treatment with OPC-21268, at dosages of 100 mg (n = 20), 200 mg (n = 20), and 300 mg/kg (n = 15), or with saline (n = 17) was started 1 hour after the induction of cold injury and was continued every 8 hours for 24 hours. Two percent Evans blue in saline (1 ml/kg) was administered intravenously before cold injury in another group of rats, 15 of which were saline-treated and 55 of which were OPC-21268-treated at the above dosages. After 24 hours, brain tissue water and electrolytes, brain tissue swelling, blood-brain barrier permeability to Evans blue, and plasma electrolytes and osmolality were determined.

RESULTS:

Compared with the saline-treated group, OPC-21268 treatment at the dosages of 200 and 300 mg/kg significantly reduced brain water content in both hemispheres (P<0.01). Swelling of the traumatized hemispheres was also significantly reduced at 200 and 300 mg/kg dosages (P<0.05). Brain tissue sodium content was significantly reduced at the dosage of 300 mg/kg (P<0.05). Blood-brain barrier permeability to Evans blue was significantly decreased in a dose-dependent manner compared with the saline-treated group (P<0.01). No significant changes were observed in other parameters.

CONCLUSION:

Our results indicate that OPC-21268 predominantly exerts a protective effect in areas where the maximum amount of blood-brain barrier breakdown occurs, and it is effective in the treatment of cold-induced vasogenic brain edema.

• Authors: I Bemana, S Nagao
• Journal: Neurosurgery (Neurosurgery) Vol. 44 Issue 1 Pg. 148-54; discussion 154-5 (Jan 1999) ISSN: 0148-396X [Print] United States
• PMID: 9894975 (Publication Type: Journal Article)

Chemical References

• Antidiuretic Hormone Receptor Antagonists
• Piperidines
• Quinolones
• OPC 21268

Topics

• Administration, Oral
• Animals
• Antidiuretic Hormone Receptor Antagonists
• Blood-Brain Barrier (drug effects)
• Brain Edema (physiopathology)
• Dose-Response Relationship, Drug
• Male
• Piperidines (pharmacology)
• Quinolones (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Water-Electrolyte Balance (drug effects)