5-[4-(2-Carboxyethylcarbamoyl)phenylazo]
salicylic acid disodium
salt dihydrate (CAS 80573-04-2, BX661A) is developed as a therapeutic agent for
ulcerative colitis. To clarify the mechanisms of action of
BX661A, the effects of
BX661A and its metabolites
5-aminosalicylic acid (5-ASA) and 4-aminobenzoyl-beta-aline (4-ABA) on polymorphonuclear (PMN) leukocyte chemotaxis and production of
reactive oxygen species (ROS) from PMN cells were investigated and compared with the effects of 2-hydroxy-5-[[4-[(2-pyridinylamino)sulfonyl]phenyl]azo]-
benzoic acid (CAS 599-79-1, SASP) and its metabolite 4-amino-N-2-pyridinyl-benzenesulfonamide (CAS 144-83-2, SP). 1.
BX661A, SASP and SP concentration-dependently inhibited guinea pig PMN cell chemotaxis induced by
zymosan-activated serum (IC50 = 1.39, 2.17 mmol/l, respectively) and by
N-formyl-methionyl-leucyl-phenylalanine (FMLP) with IC50 values of 0.55, 0.06 and 0.66 mmol/l, respectively. 5-ASA and
4-ABA weakly affected the PMN cell chemotaxis induced by
zymosan-activated serum (both IC50 values > or = 10 mmol/l) and by FMLP (IC50 > or = 10 and 8.05 mmol/l, respectively). 2.
BX661A, SASP and SP concentration-dependently inhibited human PMN cell chemotaxis induced by FMLP with IC50 values of 0.68, 0.05 and 2.68 mmol/l, respectively, but both IC50 values of 5-ASA and
4-ABA were > 10 mmol/l. 3.
BX661A, SASP, 5-ASA,
4-ABA and SP inhibited ROS production from rat PMN cells stimulated by FMLP in a concentration-dependent manner (IC50 = 58.4, 27.5, 0.61, 1242 and 13.9 mmol/l, respectively). 4.
BX661A, SASP, 5-ASA,
4-ABA and SP inhibited ROS production from human PMN cells stimulated by FMLP in a concentration-dependent manner (IC50 = 67.4, 46.1, 0.69, 748 and 8.31 mumol/l, respectively). These results suggest that
BX661A itself has inhibitory effects against PMN cell chemotaxis and ROS production from PMNs and that 5-ASA, which is the active moiety of
BX661A, has a potent inhibitory effect against ROS production from PMNs. Therefore, these effects may be partially involved in the
therapeutic effects of
BX661A on
ulcerative colitis.