In vivo effect of 5-ethynyluracil on 5-fluorouracil metabolism determined by 19F nuclear magnetic resonance spectroscopy.

Abstract

Biochemical modulation of 5-fluorouracil (5-FU) has been used over the past 20 years to improve the therapeutic efficacy of this antineoplastic agent. Recently, modulation of the catabolic pathway of this fluoropyrimidine has been the focus of extensive preclinical and clinical investigation. Dihydropyrimidine dehydrogenase catalyzes the rate-limiting step in the catabolism of 5-FU and rapidly degrades 60-90% of the drug. An irreversible inactivating inhibitor of this enzyme, 5-ethynyluracil (EU), markedly improves the antitumor effect of 5-FU in animal models. Early clinical studies have shown a substantial alteration of the systemic disposition of 5-FU with an increase in 5-FU terminal half-life and have also indicated that EU allows safe oral administration of 5-FU by improving the oral bioavailability of the fluoropyrimidine, which is otherwise too erratic and unpredictable for a drug with such a limited therapeutic window. We evaluated the effect of EU on the metabolism of 5-FU in mice bearing colon 38 tumors using 19F nuclear magnetic resonance spectroscopy. Ex vivo measurements of tissue extracts from liver, kidney, and tumor indicated a >95% elimination of alpha-fluoro-beta-ureidopropionic acid and a-fluoro-beta-alanine signals in the tissues of mice that received 2 mg/kg of EU before administration of 5-FU. The spectra also showed an increased formation of fluoronucleotides in both normal and tumor tissues, a prolonged presence of 5-FU, and the accumulation of 5-fluorouridine that otherwise is undetectable, particularly in normal tissues. The in vivo NMR experiments on colon 38 tumors confirmed these findings, showing a complete elimination of the a-fluoro-beta-ureidopropionic acid and a-fluoro-beta-alanine signals in tumors treated with EU and a dramatic formation and accumulation of 5-fluorouridine mono-, di-, and triphosphates and 5-fluorouridine. Thus, by inactivating dihydropyrimidine dehydrogenase, EU prolonged the half-life for 5-FU, almost completely eliminated its catabolism for 4-6 h, which led to an increased accumulation of 5-fluorouridine mono-, di-, and triphosphates in both normal and tumor tissues.

• Authors: E R Adams, J J Leffert, D J Craig, T Spector, G Pizzorno
• Journal: Cancer research (Cancer Res) Vol. 59 Issue 1 Pg. 122-7 (Jan 01 1999) ISSN: 0008-5472 [Print] United States
• PMID: 9892196 (Publication Type: Journal Article)

Chemical References

• Enzyme Inhibitors
• eniluracil
• Uracil
• Oxidoreductases
• Dihydrouracil Dehydrogenase (NADP)
• Fluorouracil

Topics

• Animals
• Dihydrouracil Dehydrogenase (NADP)
• Enzyme Inhibitors (pharmacology, therapeutic use)
• Fluorouracil (analysis, metabolism)
• Magnetic Resonance Spectroscopy
• Mice
• Mice, Inbred C57BL
• Neoplasms, Experimental (drug therapy, metabolism)
• Oxidoreductases (antagonists & inhibitors)
• Uracil (analogs & derivatives, pharmacology, therapeutic use)