Abstract | BACKGROUND: STUDY DESIGN/MATERIALS AND METHODS: The rat common carotid artery was balloon-injured, pressurized at 400 mm Hg for 2 minutes with the photosensitizer dye benzoporphyrin-derivative (BPD), and irradiated with 690 nm laser light at a fluence of 100 J/cm2. Control animals were pressurized with saline only, or received no additional treatment than balloon-injury. RESULTS: Pressurization with BPD resulted in complete penetration of the intima and media and was associated with relatively high tissue, but almost no detectable serum BPD concentrations. No skin photosensitization or other systemic side effects were observed with photosensitizer administration. After 9 days, PDT-treated arteries displayed a significantly lower number of smooth muscle cells in the arterial wall than balloon-injured (P < 0.001) or saline-pressurized arteries (P < 0.0002), and no intimal hyperplasia. At 21 days, IH after PDT was significantly reduced as compared with balloon-injured (P < 0.0004), or saline-pressurized arteries (P < 0.003) with no arterial dilatation. CONCLUSIONS: Site-specific delivery of liposomal BPD followed by PDT represents a safe method to treat arteries, and may be effectively used in vivo to inhibit the development of intimal hyperplasia.
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Authors | F Adili, R G Statius van Eps, T J Flotte, G M LaMuraglia |
Journal | Lasers in surgery and medicine
(Lasers Surg Med)
Vol. 23
Issue 5
Pg. 263-73
( 1998)
ISSN: 0196-8092 [Print] United States |
PMID | 9888322
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Photosensitizing Agents
- Porphyrins
- Verteporfin
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Topics |
- Animals
- Carotid Arteries
(drug effects, metabolism, pathology)
- Drug Evaluation, Preclinical
- Hyperplasia
(drug therapy, metabolism, pathology)
- Immunohistochemistry
- Injections, Intralesional
(methods)
- Laser Therapy
- Male
- Photochemotherapy
(methods)
- Photosensitizing Agents
(administration & dosage, pharmacokinetics)
- Porphyrins
(administration & dosage, pharmacokinetics)
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Tunica Intima
(drug effects, metabolism, pathology)
- Verteporfin
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