Abstract |
Measurement of groups of neuronal activities following pelvic nerve transection to the major pelvic ganglion (MPG) of the rat was performed using voltage-sensitive dye ( RH795) and an optical recording system. In control MPG, averaged neuron diameters were 32.0 +/- 0.6 x 22.6 +/- 0.4 microm. Application of KCl (10-50 mM) to the ganglia exhibited excitation which increased in a dose-dependent fashion. Fluctuating membrane potentials were not observed in control ganglion neurons. After the denervation of pelvic nerve chronically (2-6 weeks), the spontaneous neuronal activities were recorded in 91% of the experiments (n = 32). The activity was occurring somewhat periodically (2-15 s). Averaged neuron diameters were 41.3 +/- 1.3 x 24.7 +/- 0.9 microm in denervated MPG which is significantly larger than control. Since average neuron size increased 4 weeks after the denervation, the new excitatory activities could have influenced the change of the neuron size. The new activities might produce contraction of target organs in the pelvic viscera.
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Authors | M Imaizumi, Y Oguma, M Kawatani |
Journal | Neuroscience letters
(Neurosci Lett)
Vol. 258
Issue 3
Pg. 159-62
(Dec 24 1998)
ISSN: 0304-3940 [Print] Ireland |
PMID | 9885955
(Publication Type: Journal Article)
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Chemical References |
- Fluorescent Dyes
- RH 795
- Styrenes
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Topics |
- Animals
- Cell Size
- Denervation
- Fluorescent Dyes
- Ganglia, Autonomic
(cytology, physiology)
- Male
- Membrane Potentials
(physiology)
- Microscopy, Fluorescence
- Neurons
(cytology, physiology)
- Pelvis
(innervation)
- Peripheral Nerves
(physiology)
- Rats
- Rats, Wistar
- Styrenes
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