Abstract | BACKGROUND: MATERIALS AND METHODS: Adult, male Sprague-Dawley rats were divided into four treatment groups: (1) sham-operated control ( laparotomy only, no ischemia), N =6; (2) ischemia control (1 h ischemia, 2 h reperfusion), N = 6; (3) intravenous infusion with OP-2507 ([15-cis-14-propylcyclohexyl]-16, 17,18,19,20-pentanor-9-deoxy-9a,6-nitrilo- PGF, methyl ether; Ono Pharmaceutical Co, Ltd, Osaka, Japan) at a dose of 1 microg/kg/min plus ischemia (1 h ischemia, 2 h reperfusion), N = 6; and (4) intravenous infusion with OP-2507 at a dose of 0.1 microg/kg/min plus ischemia (1 h ischemia, 2 h reperfusion), N = 6. A laser-Doppler flowmeter and in vivo microscopy were used to investigate hepatic microcirculation. Tissue malondialdehyde (MDA) and adenosine triphosphate ( ATP) levels were determined at the end of the experiment. RESULTS: Compared with ischemia alone, OP-2507 significantly reduced the extent of microcirculatory and hemodynamic derangement following ischemia-reperfusion. The changes of mean systolic arterial pressure (MSAP) following ischemia-reperfusion showed biphasic alterations. OP-2507 at both doses significantly attenuated decreases in MSAP. OP-2507 lessened adherent leukocyte count and improved flow velocity in the sinusoids and postsinusoidal venules. OP-2507 at the dose of 1 microg/kg/min reduced MDA (1.04 +/- 0.27 micromol/ g protein vs 2.64 +/- 0.59 micromol/ g protein in the ischemia and reperfusion group) and increased ATP levels (2.03 +/- 0.17 micromol/g wet wt vs 0.73 +/- 0.21 micromol/g wet wt in the ischemia and reperfusion group), while OP-2507 at a smaller dose (0. 1 microg/kg/min) had lesser but significant effects on MDA and ATP alterations. CONCLUSION:
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Authors | H M Chen, M F Chen, M H Shyr |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 80
Issue 2
Pg. 333-8
(Dec 1998)
ISSN: 0022-4804 [Print] United States |
PMID | 9878334
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 1998 Academic Press. |
Chemical References |
- Malondialdehyde
- OP 2507
- Adenosine Triphosphate
- Epoprostenol
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Topics |
- Adenosine Triphosphate
(metabolism)
- Animals
- Blood Flow Velocity
(drug effects)
- Blood Pressure
(drug effects)
- Disease Models, Animal
- Energy Metabolism
(drug effects)
- Epoprostenol
(administration & dosage, analogs & derivatives, pharmacology)
- Infusions, Intravenous
- Leukocytes
(drug effects, physiology)
- Lipid Peroxidation
(drug effects)
- Liver
(blood supply, drug effects, injuries)
- Liver Circulation
(drug effects)
- Male
- Malondialdehyde
(metabolism)
- Microcirculation
(drug effects)
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(drug therapy, metabolism, physiopathology)
- Time Factors
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