Beneficial effect of octreotide treatment in acute pancreatitis in rats.

Abstract

CONCLUSIONS:

Octreotide treatment contributes to the regulation of tumor necrosis factor (TNF) production in sodium taurocholate-induced acute necrotizing pancreatitis in rats. Owing to its complex effect, octreotide can partially ameliorate the deleterious consequences of acute necrotizing pancreatitis. Elevated TNF and interleukin-6 (IL-6) levels in the peritoneal fluid may be considered a consequence of the activation of peritoneal macrophages.

BACKGROUND:

The effects of octreotide on exocrine pancreatic function have been investigated in numerous studies, but little attention has been paid to its influence on cytokine production in acute pancreatitis.

METHODS:

Acute pancreatitis was induced by the retrograde injection of taurocholic acid into the pancreatic duct in male Wistar rats. Serum amylase activity, wet pancreatic weight/body weight (pw/bw) ratio, and TNF and IL-6 levels were measured. Four micrograms/kg of octreotide was administered subcutaneously at the time of induction of pancreatitis and 24 or 48 h later. Rats were sacrificed 6, 24, 48, or 72 h after the operation.

RESULTS:

The serum amylase level and pancreatic weight to body weight ratio were decreased significantly in the octreotide-treated group. The serum TNF level was decreased significantly in the octreotide-treated group as compared with the control group at 6, 24, and 48 h (0.6 +/- 1.5, 2.0 +/- 3.3, and 0 vs 50 +/- 15.5, 37.5 +/- 18.4, and 13.1 +/- 12.5 U/mL, respectively). The ascites TNF level was decreased to 0 in the octreotide-treated group and was elevated in the control group at 72 h (28.0 +/- 49.0 U/mL). IL-6 production in ascites was extremely high in both groups at 6 h (80,000 +/- 43,817 pg/mL and 58,500 +/- 33,335 pg/mL), but the difference was not significant.

• Authors: J Marton, Z Szasz, Z Nagy, K Jarmay, T Takacs, J Lonovics, A Balogh, G Farkas
• Journal: International journal of pancreatology : official journal of the International Association of Pancreatology (Int J Pancreatol) Vol. 24 Issue 3 Pg. 203-10 (Dec 1998) ISSN: 0169-4197 [Print] United States
• PMID: 9873955 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Gastrointestinal Agents
• Interleukin-6
• Tumor Necrosis Factor-alpha
• Taurocholic Acid
• Amylases
• Octreotide

Topics

• Amylases (blood)
• Animals
• Ascitic Fluid (metabolism)
• Disease Models, Animal
• Gastrointestinal Agents (therapeutic use)
• Interleukin-6 (blood, metabolism)
• Macrophage Activation
• Male
• Octreotide (therapeutic use)
• Pancreatitis, Acute Necrotizing (drug therapy, etiology, physiopathology)
• Rats
• Rats, Wistar
• Taurocholic Acid (administration & dosage, toxicity)
• Tumor Necrosis Factor-alpha (metabolism)