Semi-automated carbohydrate-deficient transferrin in primary biliary cirrhosis: a pilot study.

Abstract

Primary biliary cirrhosis (PBC) is one of the few non-alcohol induced liver pathologies which causes false positive results in the evaluation of carbohydrate-deficient transferrin (CDT) for the diagnosis of alcohol misuse. This phenomenon has only been observed when using the CDTect assay (Pharmacia & Upjohn, Uppsala, Sweden). In this study, we evaluated CDT in female PBC patients (n = 14) by a new CDT procedure, the %CDT turbidimetric immunoassay (TIA, Axis Biochemicals, Oslo, Norway) using the isoelectric focusing/immunoblotting/laser densitometry (IEF/IB/LD, Specialty Laboratories, Santa Monica, CA, USA) procedure as the gold standard. One of the PBC patients tested CDT+ by IEF/IB/LD (cut-off >9 densitometry units, DU) and %CDT TIA (cut off >6%); one patient tested at the cut-off point of the IEF/IB/LD and another one tested at the cut-off point of the %CDT TIA. Thus, unlike CDTect, the %CDT TIA is a procedure that produces few false positives in PBC.

• Authors: P Bean, A Husa, K Liegmann, E Sundrehagen
• Journal: Alcohol and alcoholism (Oxford, Oxfordshire) (Alcohol Alcohol) 1998 Nov-Dec Vol. 33 Issue 6 Pg. 657-60 ISSN: 0735-0414 [Print] England
• PMID: 9872356 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• Transferrin
• carbohydrate-deficient transferrin

Topics

• Adult
• Aged
• Alcoholism (blood)
• Biomarkers (blood)
• False Positive Reactions
• Female
• Humans
• Liver Cirrhosis, Biliary (blood)
• Middle Aged
• Transferrin (analogs & derivatives, analysis)