Inhibitory effect of ibudilast (KC-404) on the expression of the beta2 integrin family on an eosinophilic cell line (EoL-1).

Abstract

BACKGROUND:

Adhesion molecules are thought to play a key role in inflammatory processes in bronchial asthma. We previously observed an increased expression of the beta2-integrin family on an eosinophilic cell line (EoL-1) by platelet-activating factor (PAF).

OBJECTIVE:

In the present study, we examined the effect of ibudilast (KC-404), a novel anti-asthma agent, on beta2 integrin expression induced by PAF.

MATERIAL AND METHODS:

EoL-1 cells (1x10(6)/mL) were incubated in the presence or absence of 10(-6) M ibudilast (KC-404), then cells were cultured in the presence or absence of PAF (10(-7) M) for 45 minutes. Flow cytometric analysis for CD11a, CD11b, and CD18 expression was examined.

RESULTS:

Ibudilast had an inhibitory effect on beta2 integrin expression induced by PAF [CD11a: 84.8% versus 73.1% (preincubation with ibudilast), CD11b: 35.8% versus 26.2%, CD18 74.9% versus 65.6%].

CONCLUSIONS:

Ibudilast (KC-404) has anti-inflammatory activities through its inhibitory effect on the expression of adhesion molecules on eosinophils.

• Authors: J Chihara, O Urayama, H Kayaba, K Honda, N Saito, N Hayashi, D Kurachi, T Yamamoto
• Journal: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology (Ann Allergy Asthma Immunol) Vol. 81 Issue 5 Pg. 448-50 (Nov 1998) ISSN: 1081-1206 [Print] United States
• PMID: 9860039 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bronchodilator Agents
• CD18 Antigens
• Integrins
• Lymphocyte Function-Associated Antigen-1
• Macrophage-1 Antigen
• Platelet Activating Factor
• Platelet Aggregation Inhibitors
• Pyridines
• ibudilast

Topics

• Asthma (drug therapy)
• Bronchodilator Agents (pharmacology)
• CD18 Antigens (biosynthesis)
• Humans
• Integrins (biosynthesis, drug effects)
• Lymphocyte Function-Associated Antigen-1 (biosynthesis)
• Macrophage-1 Antigen (biosynthesis)
• Platelet Activating Factor (pharmacology)
• Platelet Aggregation Inhibitors (pharmacology)
• Pyridines (pharmacology)
• Tumor Cells, Cultured (metabolism)