[Effect of matrix metalloproteinase inhibitor on experimental pseudomonal corneal ulceration].

Abstract

We examined the effect of matrix metalloproteinase (MMP) inhibitor (CS-610) on experimental pseudomonal corneal ulceration by clinical and histological evaluation. Intrastromal injection of 3.5 microliters sterile culture broth of P. aeruginosa, IID-1117 (13.5 unit Type I collagenase equivalent proteinase activities), was done to induce corneal ulcers in guinea pigs. The animals were divided into two groups of 23 each. The CS-610 group received topical CS-610 (400 micrograms/ml) treatment at 2-hour intervals and the control group received only the vehicle of CS-610 at the same intervals. In the control group, corneas developed acute corneal damage following corneal ulcerations at 6-12 hours. In the CS-610 group, these corneal lesions were inhibited in most of the eyes (p < 0.01). In the late period, as inflammatory cells migrated into the cornea, some animals of the CS-610 group developed corneal ulcer. The results indicated that CS-610 had a potent inhibitory activity against pseudomonal proteinases in vivo. The results also suggested that the mechanism of the ulceration model involved not only pseudomonal proteinases but also endogenous responses.

• Authors: K Kosaku, N Maeda, T Yokoyama, T Inoue, Y Yoshida, T Hosokawa, M Sawa
• Journal: Nippon Ganka Gakkai zasshi (Nippon Ganka Gakkai Zasshi) Vol. 102 Issue 11 Pg. 717-23 (Nov 1998) ISSN: 0029-0203 [Print] Japan
• PMID: 9852715 (Publication Type: English Abstract, Journal Article)

Chemical References

• CS 610
• Enzyme Inhibitors
• Indoles
• Metalloendopeptidases

Topics

• Animals
• Cornea (pathology)
• Corneal Ulcer (drug therapy, etiology)
• Disease Models, Animal
• Enzyme Inhibitors (therapeutic use)
• Guinea Pigs
• Indoles (therapeutic use)
• Keratitis (complications, microbiology)
• Male
• Metalloendopeptidases (antagonists & inhibitors)
• Pseudomonas Infections