Glutathione S-transferase pi immunostaining of cisplatin-resistant ovarian cancer cells in ascites.

Abstract

OBJECTIVE:

To clarify immunohistochemically the correlation between glutathione S-transferase pi (GST pi) expression of surgically resected specimens and clinical response in ovarian cancer and to evaluate ascites cytology using GST pi staining.

STUDY DESIGN:

Eighty-seven patients with ovarian cancer underwent initial debulking surgery and received cisplatin-based chemotherapy after surgery. Immunostaining for GST pi was performed on formalin-fixed sections of the patients' tumors. The cytologic slides of 24 cases were acquired for evaluation of GST pi staining.

RESULTS:

Of 87 surgically resected specimens, 55 (63.2%) were GST pi positive. Twenty-five of 28 patients (89.3%) who showed no response to chemotherapy had GST pi-positive tumor cells. The predictive value of positive GST pi staining for drug resistance was 75.8% (25/33). Of 18 cases that were GST pi positive in surgically resected specimens, 17 were positive in ascites cytology. Five cases were negative in both resected specimens and ascites cytology. There was a significant correlation in the GST pi labelling index between resected specimen and ascites cytology from the same case; the correlation coefficient was .701 and P value < .001.

CONCLUSION:

Overexpression of GST pi is related to resistance to cisplatin, and GST pi staining of ascites cytology can be used in pretherapeutic assessment of patients with ovarian cancer.

• Authors: H Kase, S Kodama, E Nagai, K Tanaka
• Journal: Acta cytologica (Acta Cytol) 1998 Nov-Dec Vol. 42 Issue 6 Pg. 1397-402 ISSN: 0001-5547 [Print] Switzerland
• PMID: 9850649 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Glutathione Transferase
• Cisplatin

Topics

• Adult
• Antineoplastic Agents (administration & dosage)
• Ascites (enzymology, pathology)
• Cisplatin (administration & dosage)
• Cystadenocarcinoma (enzymology, pathology, surgery)
• Drug Resistance, Neoplasm
• Female
• Glutathione Transferase (analysis, immunology)
• Humans
• Immunohistochemistry
• Middle Aged
• Ovarian Neoplasms (enzymology, pathology, surgery)