Identification of a proline-binding motif regulating CD2-triggered T lymphocyte activation.

Abstract

An intracellular protein termed CD2 binding protein 2 (CD2BP2), which binds to a site containing two PPPGHR segments within the cytoplasmic region of CD2, was identified. Mutagenesis and NMR analysis demonstrated that the CD2 binding region of CD2BP2 includes a 17-aa motif (GPY[orF]xxxxM[orV]xxWxxx GYF), also found in several yeast and Caenorhabditis elegans proteins of unknown function. In Jurkat T cells, over-expression of the isolated CD2BP2 domain binding to CD2 enhances the production of interleukin 2 on crosslinking of CD2 but not the T cell receptor. Hence, a proline-binding module distinct from SH3 and WW domains regulates protein-protein interactions.

• Authors: K Nishizawa, C Freund, J Li, G Wagner, E L Reinherz
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 95 Issue 25 Pg. 14897-902 (Dec 08 1998) ISSN: 0027-8424 [Print] United States
• PMID: 9843987 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Adaptor Proteins, Signal Transducing
• CD2 Antigens
• CD2BP2 protein, human
• Carrier Proteins
• Proline

Topics

• Adaptor Proteins, Signal Transducing
• Amino Acid Sequence
• Animals
• Base Sequence
• Binding Sites
• CD2 Antigens (genetics, immunology)
• Caenorhabditis elegans
• Carrier Proteins (genetics, immunology)
• DNA Mutational Analysis
• Humans
• Lymphocyte Activation
• Molecular Sequence Data
• Proline
• Saccharomyces cerevisiae
• Sequence Analysis
• T-Lymphocytes (immunology)