Abstract |
To characterize human herpesvirus 8 (HHV-8) gB, the open reading frame was PCR amplified from the HHV-8-infected cell line BCBL-1 and cloned into an expression vector. To facilitate detection of expressed HHV-8 gB, the cytoplasmic tail of the glycoprotein was tagged with the influenza hemagglutinin (HA) epitope. Expression of tagged HHV-8 gB (gB-HA), as well as the untagged form, was readily detected in CHO-K1 cells and several lymphoblastoid cell lines (LCLs). HHV-8 gB-HA was sensitive to endoglycosidase H treatment, and immunofluorescence revealed that HHV-8 gB-HA was detectable in the perinuclear region of CHO-K1 cells. These observations suggest that HHV-8 gB is not processed in the Golgi and localizes to the endoplasmic reticulum or nuclear membrane. Because both HHV-8 and EBV are gamma-herpesviruses, the ability of HHV-8 gB to interact with and functionally complement EBV gp110 was examined. HHV-8 gB-HA and EBV gp110 co-immunoprecipitated, indicating formation of hetero-oligomers. However, HHV-8 gB-HA and HHV-8 gB failed to restore the infectivity of gp110-negative EBV mutants. These findings indicate that although HHV-8 gB and EBV gp110 have similar patterns of intracellular localization and can interact, there is not sufficient functional homology to allow efficient complementation.
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Authors | P E Pertel, P G Spear, R Longnecker |
Journal | Virology
(Virology)
Vol. 251
Issue 2
Pg. 402-13
(Nov 25 1998)
ISSN: 0042-6822 [Print] United States |
PMID | 9837804
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Copyright | Copyright 1998 Academic Press. |
Chemical References |
- DNA, Viral
- Epstein-Barr virus glycoprotein gp110
- Viral Envelope Proteins
- Viral Proteins
- glycoprotein B, Simplexvirus
- glycoprotein B, human herpesvirus 7
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Topics |
- Amino Acid Sequence
- Animals
- Blotting, Western
- CHO Cells
- Cricetinae
- DNA, Viral
(genetics)
- Glycosylation
- Herpesvirus 4, Human
(genetics, pathogenicity)
- Herpesvirus 8, Human
(genetics, metabolism)
- Humans
- Molecular Sequence Data
- Open Reading Frames
- Viral Envelope Proteins
(chemistry, genetics, metabolism)
- Viral Proteins
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