Abstract |
Experimental autoimmune neuritis (EAN) is a CD4+ T cell-mediated, inflammatory demyelinating disease of the peripheral nervous system (PNS) that serves as a model for Guillain-Barré syndrome (GBS) in humans. Various mouse and rat strains show different susceptibilities to EAN that can be induced by immunization with bovine PNS myelin (BPM) + Freund's complete adjuvant (FCA). We examined PNS-induced T and B cell responses and cytokine protein production as well as mRNA expression to study the mechanisms behind susceptibility to EAN in Lewis rats and resistance in Sprague-Dawley (SD) rats. Lewis rats with EAN have elevated PNS myelin-reactive interferon-gamma (IFN-gamma) production, TNF-alpha mRNA expression, and increased B cell responses to PNS myelin antigens, but low PNS myelin-reactive transforming growth factor-beta ( TGF-beta) and interleukin (IL)-10 mRNA expression in lymph node mononuclear cells (MNC). In contrast, resistance to EAN in SD rats is associated with reduced BPM and P2 peptide-reactive IFN-gamma production, TNF-alpha mRNA expression, and suppressed B cell responses to PNS myelin antigens as well as up-regulation of TGF-beta and IL-10 mRNA expression. Resistance to EAN is also associated with low-grade inflammation or absence of histological evidence of EAN. These results suggest that differential autoreactive T and B cells responses to PNS myelin antigens are strain specific, and the susceptibility to EAN is related to quantitative rather than qualitative differences in distribution between proinflammatory and anti-inflammatory cytokines.
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Authors | J Zhu, L P Zou, M Bakhiet, E Mix |
Journal | Journal of neuroscience research
(J Neurosci Res)
Vol. 54
Issue 3
Pg. 373-81
(Nov 01 1998)
ISSN: 0360-4012 [Print] United States |
PMID | 9819142
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- Immunoglobulin G
- Myelin P2 Protein
- Phytohemagglutinins
- Receptors, Cholinergic
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Topics |
- Animals
- B-Lymphocytes
(immunology, metabolism)
- Body Weight
- Cytokines
(genetics)
- Disease Susceptibility
- Immunoglobulin G
(immunology)
- In Situ Hybridization
- Lymph Nodes
(immunology, metabolism)
- Macrophages
(metabolism)
- Male
- Myelin P2 Protein
(immunology)
- Myelin Sheath
(immunology)
- Neuritis, Autoimmune, Experimental
(etiology, immunology)
- Phytohemagglutinins
(pharmacology)
- Rats
- Rats, Inbred Lew
- Rats, Sprague-Dawley
- Receptors, Cholinergic
(immunology)
- Sciatic Nerve
(immunology, pathology)
- T-Lymphocytes
(immunology, metabolism)
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