The Escherichia coli cmlA gene encodes the multidrug efflux pump Cmr/MdfA and is responsible for isopropyl-beta-D-thiogalactopyranoside exclusion and spectinomycin sensitivity.

Abstract	Expression of cloned genes from isopropyl-beta-D-thiogalactopyranoside (IPTG)-regulated promoters is lowered when the Escherichia coli CmlA/Cmr/MdfA efflux pump is overexpressed, probably due to IPTG exclusion from the cytoplasm. The previously reported cmlA1 mutation confers a similar phenotype. cmlA1 contains an IS30 insertion upstream of cmr/mdfA, which creates a putative promoter. CmlA overproduction also causes spectinomycin hypersensitivity.
Authors	C Bohn, P Bouloc
Journal	Journal of bacteriology (J Bacteriol) Vol. 180 Issue 22 Pg. 6072-5 (Nov 1998) ISSN: 0021-9193 [Print] United States
PMID	9811673 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Bacterial Proteins Carrier Proteins Escherichia coli Proteins Mdfa protein, E coli Membrane Proteins Membrane Transport Proteins Isopropyl Thiogalactoside Spectinomycin beta-Galactosidase
Topics	Alleles Bacterial Proteins Carrier Proteins (genetics, metabolism) Drug Resistance, Microbial (genetics) Escherichia coli (genetics) Escherichia coli Proteins Gene Expression Isopropyl Thiogalactoside (metabolism) Membrane Proteins (genetics, metabolism) Membrane Transport Proteins Mutagenesis Plasmids Spectinomycin (metabolism) beta-Galactosidase (biosynthesis)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!