Increased metabolism of peroxynitrite-modified [125I] beta-VLDL by monocytes from hypercholesterolemic rabbits.

Abstract

The metabolism (uptake and degradation) of peroxynitrite-modified beta-VLDL and native beta-VLDL labeled with [125I]iodine was studied in monocytes isolated from peripheral blood of hypercholesterolemic (HC) and normolipidemic rabbits (NC). The peroxynitrite-modified beta-VLDL uptake and degradation was up to 2-fold higher in monocytes from HC rabbits than monocytes from NC rabbits. In addition, monocytes from HC rabbits took up and degraded significantly more peroxynitrite-modified beta-VLDL than native beta-VLDL. In contrast, monocytes from NC rabbits took up both lipoproteins in a similar rate and degraded more native beta-VLDL than peroxynitrite-modified beta-VLDL. Our results suggest that hypercholesterolemia can affect monocyte metabolism enhancing foam cell formation by increasing the influx of peroxynitrite-modified beta-VLDL.

• Authors: E L da Silva, D S Abdalla
• Journal: Biochemistry and molecular biology international (Biochem Mol Biol Int) Vol. 46 Issue 2 Pg. 343-53 (Oct 1998) ISSN: 1039-9712 [Print] England
• PMID: 9801802 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cholesterol, Dietary
• Lipoproteins, VLDL
• Nitrates
• Triglycerides
• peroxynitric acid
• Cholesterol

Topics

• Animals
• Cholesterol (blood)
• Cholesterol, Dietary (administration & dosage)
• Hypercholesterolemia (blood)
• Lipoproteins, VLDL (chemistry, metabolism)
• Male
• Monocytes (metabolism)
• Nitrates (pharmacology)
• Rabbits
• Triglycerides (blood)