The aim of the present work was to investigate the
biological hazard of photooxidation products of
m-chloroperbenzoic acid (
mCPBA), as a novel photo-sensitizer, on lysis and
membrane lipid peroxidation of human red blood cells (RBC). The photohemolysis activity of
mCPBA oxidative products was concentration- and exposure time-dependent. Ten minutes photoexposure time and 100 micromol/L of
mCPBA concentration were optimum to study the effect of generated
superoxide (O2.-) and
hydroxyl (.
OH) radicals on RBC. The hemolytic effect of
mCPBA was highly significantly inhibited by
formate (as an .
OH radical scavenger) compared with the partial inhibition effect of SOD-like Cu(II) complex (as O2.- radical Scavenger). The MDA value (an end product of
membrane lipid peroxidation of RBC) induced by
mCPBA was highly significantly decreased by
formate. The generation of O2.- radicals by
mCPBA was also confirmed by the partial hemolytic effect of
phenazine methosulfate (PMS., O2.- radical generation). The data suggest the molecular mechanism of the
oxygen radical species (O2.- and .
OH through the
photosensitization reaction of
mCPBA and explain that
hydroxyl radicals (.
OH) play an active role in the photohemolysis process and peroxidation of
membrane lipids of human erythrocytes.