Abstract | BACKGROUND: AIMS: To compare FGF-5 expression in the normal pancreas and in the pancreas of patients with chronic pancreatitis (CP) and to characterise FGF-5 expression and secretion in TAKA-1 cells, an immortalised Syrian hamster pancreatic duct cell line. METHODS AND RESULTS: Northern blotting revealed the presence of a 4.0 kb FGF-5 mRNA transcript in both normal and CP tissue samples. Densitometric analysis indicated that the transcript levels were increased by a factor of 1.44 in CP tissue samples compared with normal tissue samples (p = 0.039). By immunohistochemisty and in situ hybridisation, FGF-5 was faintly expressed in ductal and islet cells in the normal pancreas. In contrast, in CP tissue samples, there was abundant expression of FGF-5 in ductal, acinar, and islet cells, as well as in periductal fibroblasts. FGF-5 was also expressed in TAKA-1 cells as determined by Northern blotting. By immunoblotting of heparinsepharose precipitates, TAKA-1 cells were shown to secrete FGF-5 into the medium. CONCLUSION: Exocrine and stromal derived FGF-5 has the potential to participate in autocrine and paracrine pathways that may contribute to the pathobiology of chronic pancreatitis.
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Authors | T Ishiwata, M Kornmann, H G Beger, M Korc |
Journal | Gut
(Gut)
Vol. 43
Issue 1
Pg. 134-9
(Jul 1998)
ISSN: 0017-5749 [Print] England |
PMID | 9771418
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- FGF5 protein, human
- RNA, Messenger
- Fibroblast Growth Factor 5
- Fibroblast Growth Factors
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Topics |
- Adult
- Aged
- Animals
- Blotting, Northern
- Chronic Disease
- Cricetinae
- Female
- Fibroblast Growth Factor 5
- Fibroblast Growth Factors
(analysis, genetics, metabolism)
- Humans
- Immunoblotting
- Immunohistochemistry
- In Situ Hybridization
- Male
- Middle Aged
- Pancreas
(metabolism)
- Pancreatitis
(metabolism)
- RNA, Messenger
(analysis)
- Tumor Cells, Cultured
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