Cytogenetic clonality analysis in monosomy 7 associated with juvenile myelomonocytic leukemia: clonality in B and NK cells, but not in T cells.

Abstract

It remains unclear which lymphoid lineages are involved in juvenile myelomonocytic leukemia (JMML). We report a JMML patient who acquired monosomy 7 after intensive chemotherapy. In this case, the expression of monosomy 7 was analyzed in T, B and natural killer (NK) cells highly purified from peripheral blood mononuclear cells of the patient. The fluorescence in situ hybridization method revealed the expression of monosomy 7 in B cells, but not T cells. Half of the NK cells expressed monosomy 7; when NK cells were divided into CD2- and CD2+ populations, this abnormality was positive in 91.1% of CD2- NK cells but in only 14.7% of CD2+ NK cells. These results suggest that, in this JMML patient who acquired monosomy 7 after intensive chemotherapy, B cells and half of NK cells, but not T cells, have monosomy 7.

• Authors: T Nakazawa, K Koike, K Agematsu, S Itoh, R Hagimoto, Y Kitazawa, T Higuchi, N Sawai, H Matsui, A Komiyama
• Journal: Leukemia research (Leuk Res) Vol. 22 Issue 10 Pg. 887-92 (Oct 1998) ISSN: 0145-2126 [Print] England
• PMID: 9766748 (Publication Type: Case Reports, Comparative Study, Journal Article)

Chemical References

• CD2 Antigens

Topics

• B-Lymphocytes
• CD2 Antigens (blood)
• Cell Lineage
• Chromosomes, Human, Pair 7
• Clone Cells
• Humans
• In Situ Hybridization, Fluorescence
• Infant, Newborn
• Karyotyping
• Killer Cells, Natural
• Leukemia, Myelomonocytic, Chronic (genetics)
• Male
• Monosomy
• T-Lymphocytes