Interferon-gamma receptor beta-chain expression and formation of alpha- and beta-chain complexes after receptor conjugation on human peripheral eosinophils.

Abstract

BACKGROUND:

It has been unclear whether or not the interferon-gamma receptor (IFN-gammaR) on human peripheral eosinophils is completely functional. Accordingly, we examined the expression of IFN-gammaR alpha- and beta-chains and the association of the two chains after binding of IFN-gamma to the receptor.

METHODS:

Peripheral blood eosinophils were obtained from 8 patients (bronchial asthma, n=6, and hypereosinophilic syndrome, n=2), and expression of the alpha- and beta-chain was investigated by flow cytometry with specific antibodies. Expression of mRNA for the alpha- and beta-subunits was also investigated by the reverse transcriptase-polymerase chain reaction.

RESULTS:

Eosinophils from all the patients were positive for both the alpha- and beta-chains by flow cytometry. In addition, mRNA for both chains was detected by the polymerase chain reaction. Furthermore, coprecipitation of the alpha- and beta-chains was only found after eosinophil stimulation with IFN-gamma.

CONCLUSIONS:

Human peripheral eosinophils apparently express both the alpha- and beta-chains of the IFN-gammaR, and the two chains may only form the receptor complex after ligand binding.

• Authors: C Ishiharu, K Ochiai, M Kagami, G Matsuyama, N Koya, H Tomioka
• Journal: International archives of allergy and immunology (Int Arch Allergy Immunol) Vol. 117 Suppl 1 Pg. 72-6 (Sep 1998) ISSN: 1018-2438 [Print] Switzerland
• PMID: 9758903 (Publication Type: Journal Article)

Chemical References

• DNA Primers
• RNA, Messenger
• Receptors, Interferon
• Recombinant Proteins
• interferon gamma receptor
• Interferon-gamma

Topics

• Adolescent
• Adult
• Asthma (blood, immunology)
• Base Sequence
• Child
• DNA Primers (genetics)
• Eosinophils (drug effects, immunology, metabolism)
• Female
• Gene Expression
• Humans
• Hypereosinophilic Syndrome (blood, immunology)
• In Vitro Techniques
• Interferon-gamma (pharmacology)
• Male
• Middle Aged
• Protein Conformation
• RNA, Messenger (blood, genetics)
• Receptors, Interferon (blood, chemistry, genetics)
• Recombinant Proteins