Abstract |
Upon two-dimensional thin-layer separation, the sulfated L-3, 4-dihydroxyphenylalanine (L-DopaS) generated enzymatically was found to co-migrate with only one of the two ninhydrin-stained spots corresponding to the two sulfated forms (3-O-sulfate and 4-O-sulfate) of synthetic L-DopaS. To clarify precisely the identity of the enzymatically generated L-DopaS, the two sulfated forms of synthetic L-DopaS were separated and purified using high performance liquid chromatography. Purified L-Dopa 3-O-sulfate and L-Dopa 4-O-sulfate were identified by 1H-nuclear magnetic resonance (NMR) spectrometry and used as standards in the analysis of the L-DopaS generated during metabolic labeling of HepG2 human hepatoma cells or enzymatic assay using recombinant human monoamine (M)-form phenol sulfotransferase. The results obtained demonstrated unequivocally the generation of L-Dopa 3-O-sulfate, indicating the specificity of the M-form phenol sulfotransferase being for the meta- hydroxyl group of L-Dopa.
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Authors | M Suiko, Y Sakakibara, R Awan-Khan, H Sakaida, H Yoshikawa, J G Ranasinghe, M C Liu |
Journal | Journal of biochemistry
(J Biochem)
Vol. 124
Issue 4
Pg. 707-11
(Oct 1998)
ISSN: 0021-924X [Print] England |
PMID | 9756614
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 3,4-dihydroxyphenylalanine 3-O-sulfate
- 3,4-dihydroxyphenylalanine 4-O-sulfate
- Isoenzymes
- Sulfates
- Sulfur Radioisotopes
- Sulfuric Acid Esters
- Levodopa
- Arylsulfotransferase
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Topics |
- Arylsulfotransferase
(metabolism)
- Carcinoma, Hepatocellular
- Chromatography, Thin Layer
- Humans
- Isoenzymes
(metabolism)
- Levodopa
(analogs & derivatives, chemistry, metabolism)
- Liver Neoplasms
- Magnetic Resonance Spectroscopy
- Substrate Specificity
- Sulfates
(metabolism)
- Sulfur Radioisotopes
- Sulfuric Acid Esters
(chemistry, metabolism)
- Tumor Cells, Cultured
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