Rat hepatic stellate cells produce cytokine-induced neutrophil chemoattractant in culture and in vivo.

Abstract	Hepatic stellate cells are widely recognized for their contribution to liver fibrosis. This study investigated whether these cells also promote hepatic inflammation by producing neutrophil chemoattractants. Specifically, stellate cells were examined as potential sources of cytokine-induced neutrophil chemoattractant (CINC), a rat chemokine resembling human interleukin-8. Stellate cells from normal rat liver expressed little or no CINC. In culture, CINC mRNA was induced rapidly, coinciding with the phenomenon of culture activation. CINC mRNA rose 4.6-fold within 3 days and was accompanied by secretion of immunoreactive and biologically active CINC protein (4.1 ng . microgram DNA-1 . day-1). Studies in vivo demonstrated that CINC could be induced in stellate cells during liver injury. CINC mRNA rose significantly (4- to 6-fold) in two models of liver disease, both of which cause stellate cell activation. In summary, the data indicate that CINC is induced during stellate cell activation in culture and in vivo. They suggest that stellate cell-derived CINC can promote hepatic inflammation in vivo.
Authors	J J Maher, J S Lozier, M K Scott
Journal	The American journal of physiology (Am J Physiol) Vol. 275 Issue 4 Pg. G847-53 (10 1998) ISSN: 0002-9513 [Print] United States
PMID	9756517 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References	CXCL1 protein, human Chemokine CXCL1 Chemokines, CXC Chemotactic Factors Cxcl1 protein, rat Cytokines Growth Inhibitors Growth Substances Intercellular Signaling Peptides and Proteins Interleukin-1 RNA, Messenger Tumor Necrosis Factor-alpha Collagen
Topics	Animals Cells, Cultured Chemokine CXCL1 Chemokines, CXC Chemotactic Factors (biosynthesis, genetics) Collagen (genetics) Cytokines (pharmacology) Growth Inhibitors (genetics) Growth Substances (biosynthesis, genetics) Humans Intercellular Signaling Peptides and Proteins Interleukin-1 (pharmacology) Kinetics Kupffer Cells (cytology, drug effects, metabolism) Liver (cytology, drug effects, metabolism) Liver Diseases (metabolism) Male RNA, Messenger (genetics) Rats Rats, Sprague-Dawley Time Factors Transcription, Genetic Tumor Necrosis Factor-alpha (pharmacology)

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