Biochemical, cellular and pharmacological activities of a human neuropeptide FF-related peptide.

Abstract

We report on the biochemical, cellular and pharmacological activities of SQA-neuropeptide FF (Ser-Gln-Ala-Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH2), a peptide sequence contained in the human neuropeptide FF (neuropeptide FF, Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH2) precursor. Quantitative autoradiography revealed that, in the superficial layers of the rat spinal cord, SQA-neuropeptide FF displayed the same high affinity for [125I]1DMe ([125I]D-Tyr-Leu-(NMe)Phe-Gln-Pro-Gln-Arg-Phe-NH2) binding sites (Ki = 0.33 nM) as did neuropeptide FF (Ki = 0.38 nM). In acutely dissociated mouse dorsal root ganglion neurones, SQA-neuropeptide FF reduced by 40% the depolarisation-induced rise in intracellular Ca2+ as measured with the Ca2+ indicator, Fluo-3. In mice, 1DMe and SQA-neuropeptide FF dose-dependently inhibited the antinociceptive effect of intracerebroventricular (i.c.v.) injections of morphine, but SQA-neuropeptide FF was less potent than 1DMe. Furthermore, SQA-neuropeptide FF, as well as 1DMe, produced marked hypothermia following third ventricle injections in mice. These data demonstrate that the human peptide, SQA-neuropeptide FF, exhibits biochemical and pharmacological properties similar to those of neuropeptide FF or neuropeptide FF analogues, and belongs to the neuropeptide FF family.

• Authors: A Gelot, H Mazarguil, P Dupuy, B Francés, C Gouardères, M Roumy, J M Zajac
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 354 Issue 2-3 Pg. 167-72 (Aug 07 1998) ISSN: 0014-2999 [Print] Netherlands
• PMID: 9754917 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Analgesics, Opioid
• Neuropeptides
• Oligopeptides
• seryl-glutaminyl-alanyl-phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide
• Morphine
• phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide
• Calcium

Topics

• Analgesics, Opioid (antagonists & inhibitors)
• Animals
• Body Temperature (drug effects)
• Calcium (metabolism)
• Ganglia, Spinal (drug effects, metabolism)
• Humans
• Hypothermia, Induced
• Male
• Mice
• Morphine (antagonists & inhibitors)
• Neurons (drug effects, metabolism)
• Neuropeptides (chemistry, pharmacology, physiology)
• Oligopeptides (chemistry, pharmacology, physiology)
• Spinal Cord (drug effects, metabolism)