Macrophage inhibition of lymphocyte and tumor cell growth is mediated by 25-hydroxycholesterol in the cell membrane.

Abstract

We have previously reported that a lipid molecule in the membrane fraction of cloned macrophage hybridomas inhibited the growth of lymphocytes and several tumor cell lines. In this study, the inhibitory lipid molecule in the membrane fraction of macrophages was analyzed by thin-layer chromatography and identified as 25-hydroxycholesterol, a family of oxysterols. This conclusion was confirmed by analysis using gas chromatography-mass spectrometry. In addition, both 25-hydroxycholesterol and the lipid molecule recovered from macrophage cell membrane induced apoptosis of the murine T cell lymphoma, BW-5147. These results suggest that an oxysterol expressed in the macrophage cell membrane may participate in the regulation of cell growth through cell contact.

• Authors: H Kato, A Horino, M Taneichi, N Fukuchi, Y Eto, H Ushijima, K Komuro, T Uchida
• Journal: International archives of allergy and immunology (Int Arch Allergy Immunol) Vol. 117 Issue 1 Pg. 78-84 (Sep 1998) ISSN: 1018-2438 [Print] Switzerland
• PMID: 9751851 (Publication Type: Journal Article)

Chemical References

• Hydroxycholesterols
• 25-hydroxycholesterol

Topics

• Animals
• Apoptosis (drug effects, immunology)
• Cell Division (immunology)
• Cell Membrane (chemistry, immunology, physiology)
• Hybridomas
• Hydroxycholesterols (chemistry, pharmacology)
• Leukemia P388 (pathology)
• Lymphocytes (drug effects, immunology)
• Macrophages (immunology)
• Mice
• Mice, Inbred Strains
• Thymoma (pathology)
• Tumor Cells, Cultured