Abstract | PURPOSE: To characterize gene expression patterns between epithelia isolated from cataractous and normal human lenses. METHODS:
Reverse transcriptase differential display was used to identify differential expression between cataractous and normal epithelia. RT-PCR was used to compare pooled and individual RNA samples. RESULTS: One transcript, up-regulated in cataractous as compared to normal epithelia, was identified as osteonectin which is also known as SPARC (secreted acidic protein rich in cysteines). RT-PCR confirmed over-expression of this RNA. High levels of osteonectin mRNA were also detected in six individual epithelia dissected from cataractous lenses. CONCLUSIONS: The present study provides evidence for up-regulation of osteonectin in human age-related cataract and suggests that osteonectin, a protein involved in cell-cycle control, extracellular matrix and Ca++ binding, plays an important role in human lens homeostasis and may be involved in processes leading to lens opacity.
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Authors | M Kantorow, J Horwitz, D Carper |
Journal | Molecular vision
(Mol Vis)
Vol. 4
Pg. 17
(Sep 17 1998)
ISSN: 1090-0535 [Electronic] United States |
PMID | 9743541
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Base Sequence
- Cataract
(metabolism)
- Female
- Humans
- Lens, Crystalline
(metabolism)
- Male
- Middle Aged
- Molecular Sequence Data
- Osteonectin
(metabolism)
- Polymerase Chain Reaction
- RNA
(analysis)
- Sequence Homology, Nucleic Acid
- Up-Regulation
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