Difference in the mechanisms for compensating ischemic acidosis in diabetic rat hearts.

Abstract

To elucidate the difference in the mechanisms for alkalization during ischemic acidosis between diabetic and non-diabetic hearts, intracellular pH (pHi) was measured by phosphorus-31 magnetic resonance spectroscopy. Diabetes was induced by the injection of streptozotocin. The accumulation of proton ion (DeltaH+) during 15 min global ischemia at 37 degreesC was calculated from pH i. There were no significant differences in DeltaH+ between diabetic (DM: 0. 54+/-0.03 micromol/l,n=6; mean+/-s.e.m.) and non-DM hearts (0.57+/-0.04, n=6), when perfused with bicarbonate buffer. However, perfusion with HEPES buffer revealed a significant increase of DeltaH+ in DM (0.85+/-0.07, n=5) compared with non-DM (0.61+/-0.06, n=5P<0.05). On the contrary, the addition of a Na+/H+ exchange inhibitor (EIPA; 1 micromol/l) to bicarbonate buffer significantly increased DeltaH+ in non-DM (1.09+/-0.10, n=4) compared with DM (0.71+/-0.03, n=5P<0.01). Perfusion with HEPES buffer and EIPA equally increased DeltaH+ in both groups (DM 1.13+/-0.13, n=4; non-DM 1.15+/-0.14, n=4). Thus, the activity of Na+/H+ exchanger during ischemic acidosis, assessed as the increase of DeltaH+ induced by addition of EIPA to bicarbonate buffer, was higher in non-DM (0.52) than DM (0.17). In contrast, the contribution of bicarbonate-dependent systems evaluated by the deference of DeltaH+ between the bicarbonate buffer and the HEPES buffer was markedly bigger in DM (0.31) than non-DM (0.04). These results indicate that Na+/H+ exchange is a major mechanism to compensate ischemic acidosis in non-DM hearts, whereas bicarbonate-dependent systems compensate the depressed activity of Na+/H+ exchange in DM.

• Authors: H Kusuoka, S Matsuda, M Ishikawa, K Koga, T Mori, H Yamaguchi, T Nishimura
• Journal: Journal of molecular and cellular cardiology (J Mol Cell Cardiol) Vol. 30 Issue 8 Pg. 1643-9 (Aug 1998) ISSN: 0022-2828 [Print] England
• PMID: 9737949 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 1998 Academic Press.

Chemical References

• Bicarbonates
• Sodium-Hydrogen Exchangers

Topics

• Acidosis (metabolism)
• Animals
• Bicarbonates (metabolism)
• Diabetes Mellitus, Experimental (complications, metabolism)
• In Vitro Techniques
• Male
• Myocardial Ischemia (complications, metabolism)
• Myocardial Reperfusion
• Rats
• Rats, Sprague-Dawley
• Sodium-Hydrogen Exchangers (metabolism)