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Immunohistochemical and serological evidence for the role of streptococcal proteinase in acute post-streptococcal glomerulonephritis.

AbstractBACKGROUND:
We have previously demonstrated the preferential secretion of streptococcal proteinase or streptococcal pyrogenic exotoxin B (SPEB) by nephritic strains of Group A streptococci isolated from the skin or throat of patients with acute poststreptococcal glomerulonephritis (APSGN).
METHODS:
To further explore the possible role of SPEB in APSGN, we performed ELISA studies to detect anti-SPEB antibodies in the sera of patients with APSGN, acute rheumatic fever (ARF), scarlet fever (SF) and normal children. Using ELISA, anti-SPEB titers on acute and convalescent APSGN sera were measured to determine immunity to APSGN. We also performed immunofluorescence studies on APSGN and non-APSGN kidney biopsies to probe for the presence and localization of SPEB.
RESULTS:
Our data show that anti-SPEB antibodies are present in APSGN sera and antibody titers are significantly higher than in ARF, SF and normal sera. Anti-SPEB titers tend to rise acutely and decrease with time but do not reach baseline after one year. When kidney biopsies were probed with rabbit anti-SPEB antibody, 12 of 18 (67%) of the APSGN cases were positive while only 4 of 25 (16%) of the non-APSGN cases were positive.
CONCLUSIONS:
In summary, we were able to demonstrate unique reactivity to SPEB in human sera and kidney biopsies of APSGN suggesting a significant role of this toxin in the pathogenesis of acute post-streptococcal glomerulonephritis.
AuthorsG A Cu, S Mezzano, J D Bannan, J B Zabriskie
JournalKidney international (Kidney Int) Vol. 54 Issue 3 Pg. 819-26 (Sep 1998) ISSN: 0085-2538 [Print] United States
PMID9734606 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Bacterial Proteins
  • Exotoxins
  • Membrane Proteins
  • SpeA protein, Streptococcus pyogenes
  • erythrogenic toxin
  • Cysteine Endopeptidases
Topics
  • Animals
  • Bacterial Proteins
  • Complement Activation
  • Cysteine Endopeptidases (physiology)
  • Exotoxins (physiology)
  • Fluorescent Antibody Technique
  • Glomerulonephritis (etiology)
  • Humans
  • Immunohistochemistry
  • Membrane Proteins
  • Rabbits
  • Rheumatic Fever (etiology)
  • Scarlet Fever (etiology)
  • Streptococcal Infections (complications)
  • Streptococcus pyogenes

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