Abstract | BACKGROUND: METHODS: To further explore the possible role of SPEB in APSGN, we performed ELISA studies to detect anti-SPEB antibodies in the sera of patients with APSGN, acute rheumatic fever (ARF), scarlet fever (SF) and normal children. Using ELISA, anti-SPEB titers on acute and convalescent APSGN sera were measured to determine immunity to APSGN. We also performed immunofluorescence studies on APSGN and non-APSGN kidney biopsies to probe for the presence and localization of SPEB. RESULTS: Our data show that anti-SPEB antibodies are present in APSGN sera and antibody titers are significantly higher than in ARF, SF and normal sera. Anti-SPEB titers tend to rise acutely and decrease with time but do not reach baseline after one year. When kidney biopsies were probed with rabbit anti-SPEB antibody, 12 of 18 (67%) of the APSGN cases were positive while only 4 of 25 (16%) of the non-APSGN cases were positive. CONCLUSIONS: In summary, we were able to demonstrate unique reactivity to SPEB in human sera and kidney biopsies of APSGN suggesting a significant role of this toxin in the pathogenesis of acute post-streptococcal glomerulonephritis.
|
Authors | G A Cu, S Mezzano, J D Bannan, J B Zabriskie |
Journal | Kidney international
(Kidney Int)
Vol. 54
Issue 3
Pg. 819-26
(Sep 1998)
ISSN: 0085-2538 [Print] United States |
PMID | 9734606
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
Chemical References |
- Bacterial Proteins
- Exotoxins
- Membrane Proteins
- SpeA protein, Streptococcus pyogenes
- erythrogenic toxin
- Cysteine Endopeptidases
|
Topics |
- Animals
- Bacterial Proteins
- Complement Activation
- Cysteine Endopeptidases
(physiology)
- Exotoxins
(physiology)
- Fluorescent Antibody Technique
- Glomerulonephritis
(etiology)
- Humans
- Immunohistochemistry
- Membrane Proteins
- Rabbits
- Rheumatic Fever
(etiology)
- Scarlet Fever
(etiology)
- Streptococcal Infections
(complications)
- Streptococcus pyogenes
|